基于强度传输方程的光子计数三维荧光成像。

IF 3.2 2区物理与天体物理 Q2 OPTICS

Optics express Pub Date : 2024-11-04 DOI:10.1364/OE.540000

Shiori Matsuda, Marin Shoda, Naru Yoneda, Manoj Kumar, Takashi Murata, Inbarasan Muniraj, Yasuhiro Awatsuji, Osamu Matoba

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引用次数: 0

摘要

针对低照度生物成像应用，提出了一种基于强度传输方程（TIE）的三维（3D）荧光成像，使用光子计数检测。实验和数值研究了利用所提议的系统实现定量相位测量所需的光子数量。结果表明，在我们的植物细胞中，重建相位信息所需的平均光子数大于每个像素约 9 个。

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Photon-counting three-dimensional fluorescence imaging based on the transport of intensity equation.

A transport of intensity equation (TIE) based three-dimensional (3D) fluorescence imaging using photon-counting detection was proposed for low light-level bioimaging applications. The number of photons required to achieve the quantitative phase measurement using the proposed system was investigated experimentally and numerically. Results show that the feasibility of reconstructing phase information with an average number of photons is greater than about 9 per pixel in our plant cells.

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来源期刊

Optics express 物理-光学

CiteScore

6.60

自引率

15.80%

发文量

5182

审稿时长

2.1 months

期刊介绍： Optics Express is the all-electronic, open access journal for optics providing rapid publication for peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.