Congcong Wang, Rui Chen, Lingxiao Gong, Hong Xu, Jie Liu, Huijuan Zhang
{"title":"利用莲子表皮酚类提取物抑制水解酶活性并减缓碳水化合物的消化。","authors":"Congcong Wang, Rui Chen, Lingxiao Gong, Hong Xu, Jie Liu, Huijuan Zhang","doi":"10.1080/14786419.2024.2426066","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigated the hypoglycaemic activity of lotus seed epicarp phenolic extract (LSEPE) by inhibiting starch hydrolytic enzyme activity and slowing down carbohydrate digestion. Total phenolic and flavonoid contents in LSEPE were 620.37 ± 10.45 mg GAE/g DW and 422.69 ± 23.57 mg RE/g DW, respectively. A total of 57 phenolic compounds were first identified in LSEPE. The IC<sub>50</sub> of LSEPE for inhibiting α-amylase and α-glucosidase were 124.44 ± 2.89 μg/mL and 0.99 ± 0.02 μg/mL, approximately 3.28 to 1.61 times that of acarbose. LSEPE acted as a mixed-type inhibitor of α-amylase and an anti-competitive-type inhibitor of α-glucosidase. UV spectroscopy, fluorescence quenching, and CD analysis showed that LSEPE could change the enzymes' microenvironment and hydrophobicity. Moreover, LSEPE slowed the starch hydrolysis rate <i>in vitro</i> and increased the proportions of slowly digestible starch (SDS) and resistant starch (RS) by 60.51% and 19.98%. Therefore, the lotus seed epicarppolyphenols could be the potential natural ingredients for developing anti-hyperglycemic functional foods.</p>","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.9000,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Inhibiting hydrolytic enzyme activity and slowing carbohydrate digestion using lotus seed epicarp phenolic extracts.\",\"authors\":\"Congcong Wang, Rui Chen, Lingxiao Gong, Hong Xu, Jie Liu, Huijuan Zhang\",\"doi\":\"10.1080/14786419.2024.2426066\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigated the hypoglycaemic activity of lotus seed epicarp phenolic extract (LSEPE) by inhibiting starch hydrolytic enzyme activity and slowing down carbohydrate digestion. Total phenolic and flavonoid contents in LSEPE were 620.37 ± 10.45 mg GAE/g DW and 422.69 ± 23.57 mg RE/g DW, respectively. A total of 57 phenolic compounds were first identified in LSEPE. The IC<sub>50</sub> of LSEPE for inhibiting α-amylase and α-glucosidase were 124.44 ± 2.89 μg/mL and 0.99 ± 0.02 μg/mL, approximately 3.28 to 1.61 times that of acarbose. LSEPE acted as a mixed-type inhibitor of α-amylase and an anti-competitive-type inhibitor of α-glucosidase. UV spectroscopy, fluorescence quenching, and CD analysis showed that LSEPE could change the enzymes' microenvironment and hydrophobicity. Moreover, LSEPE slowed the starch hydrolysis rate <i>in vitro</i> and increased the proportions of slowly digestible starch (SDS) and resistant starch (RS) by 60.51% and 19.98%. Therefore, the lotus seed epicarppolyphenols could be the potential natural ingredients for developing anti-hyperglycemic functional foods.</p>\",\"PeriodicalId\":18990,\"journal\":{\"name\":\"Natural Product Research\",\"volume\":\" \",\"pages\":\"1-12\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-11-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Natural Product Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1080/14786419.2024.2426066\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/14786419.2024.2426066","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Inhibiting hydrolytic enzyme activity and slowing carbohydrate digestion using lotus seed epicarp phenolic extracts.
This study investigated the hypoglycaemic activity of lotus seed epicarp phenolic extract (LSEPE) by inhibiting starch hydrolytic enzyme activity and slowing down carbohydrate digestion. Total phenolic and flavonoid contents in LSEPE were 620.37 ± 10.45 mg GAE/g DW and 422.69 ± 23.57 mg RE/g DW, respectively. A total of 57 phenolic compounds were first identified in LSEPE. The IC50 of LSEPE for inhibiting α-amylase and α-glucosidase were 124.44 ± 2.89 μg/mL and 0.99 ± 0.02 μg/mL, approximately 3.28 to 1.61 times that of acarbose. LSEPE acted as a mixed-type inhibitor of α-amylase and an anti-competitive-type inhibitor of α-glucosidase. UV spectroscopy, fluorescence quenching, and CD analysis showed that LSEPE could change the enzymes' microenvironment and hydrophobicity. Moreover, LSEPE slowed the starch hydrolysis rate in vitro and increased the proportions of slowly digestible starch (SDS) and resistant starch (RS) by 60.51% and 19.98%. Therefore, the lotus seed epicarppolyphenols could be the potential natural ingredients for developing anti-hyperglycemic functional foods.
期刊介绍：
The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds.
The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal.
Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section.
All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
