哮喘患者活化嗜酸性粒细胞 mRNA 表达与空气污染物暴露之间的相关性

IF 3.1 4区医学 Q3 IMMUNOLOGY

Immunity, Inflammation and Disease Pub Date : 2024-11-22 DOI:10.1002/iid3.70065

Ting-Yu Lin, Po-Jui Chang, Chun-Yu Lo, Hsiao-Chi Chuang, Chung-Shu Lee, Chih-Hao Chang, Chih-Teng Yu, Meng-Heng Hsieh, Chien-Ying Liu, Chih-Hsi Scott Kuo, Shu-Min Lin

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引用次数: 0

摘要

背景：嗜酸性粒细胞活化与哮喘有关：嗜酸性粒细胞活化与哮喘有关。空气污染是否会影响哮喘患者血液中嗜酸性粒细胞的活化仍是未知数。在这项研究中，我们调查了接受全球哮喘倡议（GINA）不同程度治疗的患者体内嗜酸性粒细胞转录活性与空气污染物暴露之间的相关性：我们评估了GINA 4或5级患者（20人）、GINA 3级患者（12人）和正常人（7人）嗜酸性粒细胞中活化和功能相关基因的表达水平；嗜酸性粒细胞被白细胞介素（IL）-5或IL-17活化。采用土地利用回归模型估算空气污染物暴露量。研究了 mRNA 表达、肺功能和空气污染物暴露之间的相关性：结果：GINA 4 或 5 患者的 TGFB1、IL7R 和 TLR3 表达水平明显高于 GINA 3 或正常人。某些基因的表达，尤其是 IL-17 激活的嗜酸性粒细胞的表达，与 GINA 4 或 5 患者的肺功能下降相关。对于 GINA 4 或 5 患者，接触 NO2 与 IL-5 激活的嗜酸性粒细胞中 TGFB1 表达上调有关。对于 GINA 3 患者，暴露于 O3 与 IL-17 活化的嗜酸性粒细胞中 CCR5、IL5RA、IL7R 和 TGFB1 表达上调以及 IL-5 活化的嗜酸性粒细胞中 IL7R 表达上调相关：结论：GINA 4 或 5 患者的嗜酸性粒细胞转录活性可能升高；这种升高与肺功能下降相关。空气污染可能会影响哮喘患者嗜酸性粒细胞 mRNA 的表达。

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Correlation Between mRNA Expression of Activated Eosinophils and Air Pollutant Exposure in Patients With Asthma

Background

Eosinophil activation is associated with asthma. Whether air pollution affects the activation of blood eosinophils in patients with asthma remains unknown. In this study, we investigated the correlation between transcriptional activity in eosinophils and air pollutant exposure in patients receiving different levels of Global Initiative for Asthma (GINA) treatment.

Methods

We evaluated the expression levels of activation- and function-related genes in eosinophils from patients with GINA 4 or 5 (n = 20), those with GINA 3 (n = 12), and normal individuals (n = 7); the eosinophils were activated with interleukin (IL)−5 or IL-17. A land use regression model was used to estimate air pollutant exposure. The correlations between mRNA expression, lung function, and air pollutant exposure were investigated.

Results

The expression levels of TGFB1, IL7R, and TLR3 were significantly higher for patients with GINA 4 or 5 than for those with GINA 3 or normal individuals. The expression of certain genes, particularly in IL-17-activated eosinophils, was correlated with lung function decline in patients with GINA 4 or 5. For patients with GINA 4 or 5, NO₂ exposure was correlated with upregulated TGFB1 expression in IL-5-activated eosinophils. For patients with GINA 3, O₃ exposure was correlated with upregulated CCR5, IL5RA, IL7R, and TGFB1 expression in IL-17-activated eosinophils and upregulated IL7R expression in IL-5-activated eosinophils.

Conclusion

Patients with GINA 4 or 5 may exhibit elevated transcriptional activity in eosinophils; this elevation is correlated with lung function decline. Air pollution may affect eosinophil mRNA expression in patients with asthma.

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来源期刊

Immunity, Inflammation and Disease Medicine-Immunology and Allergy

CiteScore

3.60

自引率

0.00%

发文量

146

审稿时长

8 weeks

期刊介绍： Immunity, Inflammation and Disease is a peer-reviewed, open access, interdisciplinary journal providing rapid publication of research across the broad field of immunology. Immunity, Inflammation and Disease gives rapid consideration to papers in all areas of clinical and basic research. The journal is indexed in Medline and the Science Citation Index Expanded (part of Web of Science), among others. It welcomes original work that enhances the understanding of immunology in areas including: • cellular and molecular immunology • clinical immunology • allergy • immunochemistry • immunogenetics • immune signalling • immune development • imaging • mathematical modelling • autoimmunity • transplantation immunology • cancer immunology