自适应调制快速波动超分辨率显微镜。

IF 3.2 2区 物理与天体物理 Q2 OPTICS
Optics express Pub Date : 2024-11-04 DOI:10.1364/OE.537728
Zhijia Liu, Longfang Yao, Li Zhang, Duantao Hou, Yiyan Fei, Lan Mi, Baoju Wang, Jiong Ma
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引用次数: 0

摘要

荧光显微镜大大推进了纳米尺度的生物成像,尤其是超分辨率显微镜(SRM)的出现,它超越了阿贝衍射极限。大多数尖端的 SR 方法都需要高精度的光学装置,这限制了 SRM 的广泛应用。基于荧光波动的 SRM(FF-SRM)无需复杂的光学元件即可突破衍射极限,因此特别适合生物成像。然而,传统的荧光波动 SRM 方法,如超分辨率光学波动成像(SOFI),仍然需要特定的荧光分子闪烁特性。与其通过寻找特定的荧光标记来增强固有的闪烁特性,不如采用空间光调制等光学方法来调整激发光场,从而更容易、更灵活地匹配导通时间比与时间随机强度波动分析。然而,尽管调制模式的具体参数对重建质量有着至关重要的影响,但尚未对其进行深入探讨。在此,我们提出了自适应调制快速波动超分辨率显微镜技术。我们的方法从理论和实验上证明,将调制单元的大小限制在一定范围内可以确保更好的图像质量,减少伪影和信号损失。我们发现该方法在应用于其他 FF-SRM 时仍然非常有效。总之,自适应调制技术的进一步发展使其行为更加稳定,并保持了高质量的成像,为基于统计分析的超分辨率成像带来了更广阔的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adaptive-modulated fast fluctuation super-resolution microscopy.

Fluorescence microscopy has significantly advanced biological imaging at the nanoscale, particularly with the advent of super-resolution microscopy (SRM), which transcends the Abbe diffraction limit. Most cutting-edge SR methods require high-precision optical setups, which constrain the widespread adoption of SRM. Fluorescence fluctuation-based SRM (FF-SRM) can break the diffraction limit without complex optical components, making it particularly well-suited for biological imaging. However, conventional FF-SRM methods, such as super-resolution optical fluctuation imaging (SOFI), still require specific fluorescent molecular blinking properties. Instead of enhancing the intrinsic blinking characteristics by finding specific fluorescent markers, employing optical methods such as spatial light modulation to adjust the excitation light field allows for easier and more flexible matching of the on-time ratio with the analysis of temporal stochastic intensity fluctuations. Nevertheless, the specific parameters of the modulation patterns have not been thoroughly explored, despite their crucial influence on the reconstruction quality. Herein, we propose adaptive-modulated fast fluctuation super-resolution microscopy. Our method demonstrates theoretically and experimentally that restricting the size of modulation units in a certain range ensures better image quality with fewer artifacts and signal losses. We find it still significantly effective when applied to other FF-SRM. Overall, the further development of the adaptive modulation technique has made it more stable in behavior and maintained high-quality imaging, presenting broader prospects for super resolution imaging based on statistical analysis.

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来源期刊
Optics express
Optics express 物理-光学
CiteScore
6.60
自引率
15.80%
发文量
5182
审稿时长
2.1 months
期刊介绍: Optics Express is the all-electronic, open access journal for optics providing rapid publication for peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.
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