在室温下通过晶体芯片进行蛋白质结晶和结构测定。

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

FEBS Open Bio Pub Date : 2024-11-21 DOI:10.1002/2211-5463.13932

Petr Pachl, Léna Coudray, Romain Vincent, Léa Nilles, Hélène Scheer, Christophe Ritzenthaler, Adéla Fejfarová, Pavlína Řezáčová, Sylvain Engilberge, Claude Sauter

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引用次数: 0

摘要

生产高质量晶体是一般晶体学研究的关键步骤，但在序列晶体学研究中，结晶条件的控制更为重要，因为序列晶体学研究需要大小和衍射特性均一的晶体集。本方案描述了一种简单易用的微流控装置的实施过程，该装置既可通过反扩散法生产晶体，也可通过序列晶体学对晶体进行原位分析。举例说明，整个程序用于根据同步辐射源在室温下收集的数据确定三种蛋白质的晶体结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Protein crystallization and structure determination at room temperature in the CrystalChip.

The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source.

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来源期刊

FEBS Open Bio BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

5.10

自引率

0.00%

发文量

173

审稿时长

10 weeks

期刊介绍： FEBS Open Bio is an online-only open access journal for the rapid publication of research articles in molecular and cellular life sciences in both health and disease. The journal''s peer review process focuses on the technical soundness of papers, leaving the assessment of their impact and importance to the scientific community. FEBS Open Bio is owned by the Federation of European Biochemical Societies (FEBS), a not-for-profit organization, and is published on behalf of FEBS by FEBS Press and Wiley. Any income from the journal will be used to support scientists through fellowships, courses, travel grants, prizes and other FEBS initiatives.