银翘丸通过 PKM2 抑制 JAK/STAT3 通路,抑制 HeLa 细胞的恶性生物学行为。

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cytotechnology Pub Date : 2025-02-01 Epub Date: 2024-11-20 DOI:10.1007/s10616-024-00668-5
Ying Shi, Xiaoli Min, Yi Li, Lihua Guo, Zheng Cai, Dongge Li, Xueying Jiang, Ni Feng, Xiaolin Li, Xiaoxia Yang
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引用次数: 0

摘要

宫颈癌是女性最常见的肿瘤之一,也是妇科健康的一大难题。研究表明,中药银翘丸能有效延缓宫颈癌的进展。因此,本研究主要探讨银翘解毒丸延缓宫颈癌进展的分子机制。通过基因表达谱交互分析(GEPIA)数据库评估了PKM2在宫颈癌中的表达水平,并通过Kaplan-Meier plotter数据库评估了PKM2基因的预后价值。用不同浓度的 YJP(2.5、5、10 和 20 毫克/毫升)处理 HeLa 宫颈癌细胞。用 ELISA 检测炎症因子的水平。细胞增殖活性、迁移和侵袭通过 CCK-8 试验、Transwell 试验和细胞划痕实验进行检测。流式细胞术检测细胞凋亡。采用 Western 印迹法检测蛋白质的表达。这项研究发现,PKM2在宫颈鳞状细胞癌(CESC)和子宫内膜腺癌组织中均上调,Kaplan-Meier分析表明,PKM2表达越高,患者生存率越低。YJP以剂量依赖的方式抑制了HeLa细胞的增殖、迁移和侵袭,促进了HeLa细胞的凋亡,并抑制了炎症因子的表达。此外,YJP 还能抑制 JAK/STAT3 通路的激活和 EMT 的发生。敲除 PKM2 也能抑制 HeLa 细胞的恶性生物学行为,但过表达 PKM2 会削弱 YJP 对 HeLa 细胞恶性生物学行为的抑制作用。JAK/STAT3通路抑制剂Angoline减弱了PKM2过表达对YJP药效的影响。总之,YJP 可以通过调节 PKM2 来抑制 JAK/STAT3 通路的激活,从而抑制 HeLa 细胞的恶性生物学行为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Yinjia pills inhibits the malignant biological behavior of HeLa cells through PKM2-medicated inhibition of JAK/STAT3 pathway.

Cervical cancer is one of the most common tumors in women and is a major problem in gynecological health. Studies have shown that Yinjia pills (YJP), a traditional Chinese medicine, can effectively slow the progression of cervical cancer. Therefore, this study mainly explored the molecular mechanism by which YJP delays the progression of cervical cancer. The expression level of PKM2 in cervical cancer was evaluated by the gene expression profiling interactive analysis (GEPIA) database, and the prognostic value of the PKM2 gene was evaluated by the Kaplan‒Meier plotter database. HeLa cervical cancer cells were treated with different concentrations of YJP (2.5, 5, 10, and 20 mg/mL). The levels of the inflammatory factors were detected by ELISA. Cell proliferation activity, migration and invasion were detected by CCK-8 assay, Transwell assays and cell scratch experiment. Apoptosis was detected by flow cytometry. Western blotting was used to detect the expression of proteins. In this study, PKM2 was upregulated in both cervical squamous cell carcinoma (CESC) and endometrial adenocarcinoma tissues, and a Kaplan‒Meier analysis showed that higher PKM2 expression was associated with lower patient survival. YJP inhibited the proliferation, migration and invasion of HeLa cells in a dose-dependent manner, promoted the apoptosis of HeLa cells, and inhibited the expression of inflammatory factors. In addition, YJP inhibited the activation of the JAK/STAT3 pathway and the occurrence of EMT. Knockdown of PKM2 also inhibited the malignant biological behavior of HeLa cells, but overexpression of PKM2 weakened the inhibitory effect of YJP on the malignant biological behavior of HeLa cells. Angoline, a JAK/STAT3 pathway inhibitor, attenuated the effect of PKM2 overexpression on the efficacy of YJP. In conclusion, YJP can inhibit the activation of the JAK/STAT3 pathway by regulating PKM2, thereby inhibiting the malignant biological behavior of HeLa cells.

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来源期刊
Cytotechnology
Cytotechnology 生物-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
49
审稿时长
6-12 weeks
期刊介绍: The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
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