自上而下的捕食者决定着土壤细菌群落的组成,而自下而上的养分则驱动着细菌的丰度。

IF 8.2 1区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Science of the Total Environment Pub Date : 2024-12-20 Epub Date: 2024-12-02 DOI:10.1016/j.scitotenv.2024.177634
Seda Ozer Bodur, Kazuki Suzuki, Naoki Harada, Rasit Asiloglu
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引用次数: 0

摘要

尽管微生物食物网中自上而下和自下而上的概念一直是生态学的主要关注点,但人们对土壤生态系统中这一概念的了解仍然较少。原生动物是细菌群落自上而下的主要捕食者,它们会改变群落的组成,而自下而上的资源则是限制细菌生长的主要因素。在这里,我们假设自上而下的捕食者会调节土壤细菌群落的组成,而自下而上的营养物质则会控制细菌的生长和数量。为了精确控制养分水平,我们使用了由煅烧粘土和沙子组合而成的惰性土壤替代品。作为对照处理,我们在微生态系统中添加了相当于参考稻田土壤的养分。为了研究碳、氮和磷的影响,我们又准备了六种自下而上的处理,分别是不添加营养元素和添加双倍营养元素的处理。每种自下而上的处理都设置了四种自上而下的处理(不添加原生动物、蓖麻原生动物、蛭形原生动物和球形异型原生动物)。共培养了 28 种处理下的 252 个微生态系统。在第 1、3 和 5 周,使用高通量测序和实时 PCR 分析细菌群落。结果显示,自上而下的捕食者显著改变了细菌群落的组成,而细菌数量主要受自下而上的营养物质控制。绝对丰度数据分析表明,自上而下和自下而上的因素都影响着细菌群落结构(群落组成和数量)。随机森林分析对与处理相关的扩增子序列变异进行了分类,结果表明,大多数相似的家族都受到自下而上和自上而下因素的影响。总之,本研究的结果完全支持我们的假设,即自上而下的捕食者改变了群落组成,而自下而上的因素影响了细菌的种群动态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Top-down predators shape soil bacterial community composition while bottom-up nutrients drive bacterial abundance.

Although the top-down and bottom-up concept in microbial food-webs has been a primary interest in ecology, less is still known about it in soil ecosystems. Protists are the primary top-down predators of bacterial communities, altering their compositions, while the bottom-up resources are the primary factors limiting bacterial growth. Here, we hypothesized that the top-down predators modulate soil bacterial community composition, while the bottom-up nutrients control the bacterial growth and population. To precisely control nutrient levels, we used an inert soil substitute consisting of a combination of calcined clay and sand. Nutrients equivalent to the reference paddy field soil were added to microcosms as a control treatment. To investigate the effects of C, N, and P, six additional bottom-up treatments in the absence and double amounts of the nutrients were prepared. Four top-down treatments (no protist addition, Acanthamoeba castellanii, Vermamoeba vermiformis, and Heteromita globosa) were set up for each bottom-up treatment. A total of 252 microcosms under 28 treatments were incubated. Bacterial communities were analyzed using high-throughput sequencing and real-time PCR in the 1st, 3rd, and 5th weeks. The results revealed that the top-down predators significantly altered the bacterial community composition, and the bacterial population was predominantly controlled by the bottom-up nutrients. Analysis of absolute abundance data demonstrated that both top-down and bottom-up factors shaped the bacterial community structure (community composition and population). Random forest analysis classified the amplicon sequence variants associated with the treatments, showing that mostly similar families were affected by both bottom-up and top-down factors. In conclusion, the results of this study fully supported our hypothesis that top-down predators alter community composition, while bottom-up factors influence bacterial population dynamics.

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来源期刊
Science of the Total Environment
Science of the Total Environment 环境科学-环境科学
CiteScore
17.60
自引率
10.20%
发文量
8726
审稿时长
2.4 months
期刊介绍: The Science of the Total Environment is an international journal dedicated to scientific research on the environment and its interaction with humanity. It covers a wide range of disciplines and seeks to publish innovative, hypothesis-driven, and impactful research that explores the entire environment, including the atmosphere, lithosphere, hydrosphere, biosphere, and anthroposphere. The journal's updated Aims & Scope emphasizes the importance of interdisciplinary environmental research with broad impact. Priority is given to studies that advance fundamental understanding and explore the interconnectedness of multiple environmental spheres. Field studies are preferred, while laboratory experiments must demonstrate significant methodological advancements or mechanistic insights with direct relevance to the environment.
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