基于智能设计的比率电化学传感器和双信号放大技术检测 microRNA-21

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-11-22 DOI:10.1016/j.aca.2024.343444

Honglei Shao, Xingming Xue, Zhaomei Sun, Xiangjiang Zheng, Pengfei Shi

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引用次数: 0

摘要

微小核糖核酸（miRNA）是早期诊断和监测癌症疾病的有效、可行的生物标志物。它在肺癌、肝癌和淋巴瘤等肿瘤细胞中高度表达。在此，我们提出了一种比率电化学传感器，利用双信号放大、杂交链反应和 Exo III 辅助放大技术对 miRNA-21 进行超灵敏检测。我们选择亚甲基蓝（MB）和血红蛋白作为两种电化学物质。结果表明，该传感器具有良好的 miRNA-21 检测性能，检测浓度范围为 1 fM 至 10 nM。值得注意的是，这种生物传感器的检测限为 0.15 fM。总之，这种 miRNA 检测策略在早期癌症筛查方面前景广阔。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Detection of microRNA-21 based on smartly designed ratiometric electrochemical sensor and dual-signal amplification

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Detection of microRNA-21 based on smartly designed ratiometric electrochemical sensor and dual-signal amplification

MicroRNA(miRNA) serves as an effective and viable biomarker for early diagnosis and monitoring of cancer disorders. It is highly expressed in tumor cells, including lung cancer, liver cancer and lymphoma. Herein, we propose a ratiometric electrochemical sensor for ultrasensitive detection of miRNA-21 using dual signal amplification, hybridization chain reaction and Exo III assisted-amplification. Methylene blue (MB) and Hemin are chosen as two electrochemical species. Then the ratiometric electrochemical sensor were developed, which showed favorable performance of miRNA-21 detection, and exhibited a detection concentration range from 1 fM to 10 nM. Notably, the limit of detection for this biosensor was 0.15 fM. Overall, this strategy for miRNA detection holds significant promise for early cancer screening.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.