微生物接种剂引起的根瘤代谢物和微生物群落变化可促进植物生长

IF 3.9 2区 农林科学 Q1 AGRONOMY
Hui Nie, Yuxuan Shi, Xinxin Yang, Jingyi Zeng, Yingzhou Tang, Xin Liu, Lianhao Sun, Yuexiang Zhou, Xian Xu, Manda Liu, Chong Li, Jinchi Zhang
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引用次数: 0

摘要

目的 微生物接种剂通过刺激植物生长,在废弃矿址的修复过程中发挥着重要作用。方法在这项研究中,我们采用了扩增子测序和液相色谱-串联质谱(LC-MS/MS)技术,通过盆栽实验研究了土壤微生物群落和代谢功能对微生物接种剂的反应。结果我们的研究结果表明,微生物接种剂显著影响了细菌和真菌群落的结构。网络分析显示,微生物接种剂提高了细菌共生网络的稳定性和复杂性,同时降低了真菌共生网络的稳定性和复杂性。此外,微生物接种剂增加了随机过程在群落组装中的重要性,尤其是在真菌群落中。代谢组分析表明,微生物接种剂显著改变了根瘤土壤的代谢特征,并上调了苯丙酮类化合物和异黄酮类化合物的代谢途径。相关分析和结构方程建模(SEM)分析进一步表明,微生物接种剂通过影响关键微生物类群以及苯丙酮类和异黄酮类化合物的代谢促进植物生长。这些结果为深入了解微生物接种剂对根瘤菌层复杂生物过程的影响提供了宝贵的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbial inoculant-induced modifications of rhizospheric metabolites and microbial communities enhance plant growth

Aims

Microbial inoculants play important roles in the remediation of abandoned mine sites by stimulating plant growth. However, further investigations are required to elucidate their impacts on soil microbial communities and metabolites in this process.

Methods

For this study, we employed amplicon sequencing and Liquid Chromatography-Tandem Mass Spectrometry (LC–MS/MS) techniques to examine the responses of soil microbial communities and metabolic functions to microbial inoculants using a pot experiment.

Results

Our findings indicated that microbial inoculants significantly influenced the structures of both bacterial and fungal communities. Network analyses revealed that microbial inoculants enhanced the stability and complexity of bacterial co-occurrence networks while reducing those of their fungal counterparts. Moreover, microbial inoculants increased the importance of stochastic processes in community assembly, particularly within fungal communities. Metabolomic analyses showed that microbial inoculants significantly modified the metabolic profiles of rhizospheric soil and upregulated metabolic pathways of phenylpropanoids and isoflavonoids. Correlation analyses and structural equation modeling (SEM) analysis further showed that microbial inoculants promote plant growth by influencing keystone microbial genera and the metabolism of phenylpropanoids and isoflavonoids.

Conclusion

Overall, this study demonstrated that microbial inoculants promote plant growth through the modification of rhizospheric keystone microbial taxa and the metabolism of phenylpropanoids and isoflavonoids. These results offer valuable insights into the complex biological processes influenced by microbial inoculants in the rhizosphere.

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来源期刊
Plant and Soil
Plant and Soil 农林科学-农艺学
CiteScore
8.20
自引率
8.20%
发文量
543
审稿时长
2.5 months
期刊介绍: Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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