用于感测 Cr3+/ClO- 的开启式 AIE 双通道荧光探针及其在细胞成像中的应用。

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2024-11-17 DOI:10.1016/j.saa.2024.125444

Huizhen Wang , Yan Tang , Kezhen Gou , Dingxin Qiu , Cheng Du , Zhengfeng Xie , Guanjun Chang

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引用次数: 0

摘要

以丹酰肼和 4,6-二甲氧基水杨醛为起始原料，合成了一种具有聚集诱导发射（AIE）特性的 Cr3+/ClO- 增强荧光探针 DNS（5-(dimethylamino)-N'-(2-hydroxy-4,6-dimethoxybenzylidene)-naphthalene-1-sulfonyl hydrazide）。该探针可在 H2O/DMSO (2:8) 溶剂体系中快速、选择性地检测 Cr3+ 和 ClO-。与 Cr3+/ClO- 结合后，探针的荧光明显增强，其他离子的干扰极小。经测定，Cr3+ 的检测限（LOD）为 5.36 × 10-7 mol/L，ClO- 的检测限（LOD）为 3.65 × 10-7 mol/L。通过约伯图、1H NMR 滴定和质谱分析，研究了 DNS 与 Cr3+/ClO- 的结合机制。此外，该探针的低细胞毒性和生物相容性表明，它具有在活细胞中检测外源 Cr3+/ClO- 和内源 ClO- 的潜力。DNS 显示了实时检测和生物成像应用的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A turn-on AIE dual-channel fluorescent probe for sensing Cr3+/ClO− and application in cell imaging

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A turn-on AIE dual-channel fluorescent probe for sensing Cr3+/ClO− and application in cell imaging

A Cr³⁺/ClO⁻-enhanced fluorescent probe, DNS (5-(dimethylamino)-N′-(2-hydroxy-4,6-dimethoxybenzylidene)-naphthalene-1-sulfonyl hydrazide), with aggregation-induced emission (AIE) properties was synthesized using dansylhydrazide and 4,6-dimethoxysalicylaldehyde as starting materials. The probe rapidly and selectively detects Cr³⁺ and ClO⁻ in a solvent system of H₂O/DMSO (2:8). Upon binding with Cr³⁺/ClO⁻, the probe exhibits a significant fluorescence enhancement, with minimal interference from other ions. The detection limits (LOD) were determined to be 5.36 × 10⁻⁷ mol/L for Cr³⁺ and 3.65 × 10⁻⁷ mol/L for ClO⁻. The binding mechanisms of DNS with Cr³⁺/ClO⁻ were investigated through Job’s plot, 1H NMR titration, and mass spectrometry. Furthermore, the probe’s low cytotoxicity and biocompatibility suggest its potential for detecting exogenous Cr³⁺/ClO⁻ and endogenous ClO⁻ in living cells. DNS shows promise for real-time detection and bioimaging applications.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.