瑞典罗讷比市大量接触全氟烷基物质的成年人对 SARS-CoV-2 mRNA 疫苗的 T 细胞反应。

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere Pub Date : 2024-11-27 DOI:10.1016/j.chemosphere.2024.143770

Axel G. Andersson , Anna Lundgren , Yiyi Xu , Christel Nielsen , Christian H. Lindh , Daniela Pineda , Josefine Vallin , Clara Johnsson , Tony Fletcher , Mats Bemark , Kristina Jakobsson , Ying Li

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引用次数: 0

摘要

背景：全氟烷基物质（PFAS）与儿童接种疫苗后抗体水平受损以及动物免疫抑制效应有关。然而，PFAS 对抗原特异性人类 T 细胞反应的体内影响尚未在成人中进行调查。在瑞典罗讷比，一个自来水厂的饮用水以前主要受到全氟己烷磺酸 (PFHxS) 和全氟辛烷磺酸 (PFOS) 的高度污染。COVID-19 疫苗接种计划为评估血清中全氟辛烷磺酸含量较高的成年人接种疫苗后的抗原特异性 T 细胞功能提供了可能性：方法：从 COVID-19 疫苗接种后 PFAS 免疫反应队列 (PIRVACoV) 中纳入 116 名来自 Ronneby 的 COVID-19 初学者和一个背景暴露组。所有参与者都接种了两剂 Spikevax® (Moderna) 疫苗。用基于 SARS-CoV-2 穗状病毒蛋白的重叠肽刺激血液中的 T 细胞，并测量它们产生的细胞因子 IFN-γ、IL-2 和 TNF。通过测定植物血凝素刺激的细胞因子和血清总免疫球蛋白水平来评估总体免疫反应。根据测量结果、地址和产前 PFAS 暴露指数进行了调整混合线性回归拟合：曾在家中使用过受污染饮用水的参与者（PFOS 47 纳克/毫升，第 5 至第 95 百分位数为 6-221 纳克/毫升）与背景组（PFOS 4 纳克/毫升，第 5 至第 95 百分位数为 2-9 纳克/毫升）之间的 PFAS 中位数血清水平差异很大。接触全氟辛烷磺酸与 SARS-CoV-2 特异性 T 细胞细胞因子反应无关（例如，测得的全氟辛烷磺酸与 IFN-γ 的关系为 +3%/四分位数之间 +3%/四分位数之间 +3%/四分位数之间 +3%）：+3%，95% 置信区间：-10, 17），也与一般免疫反应无关：本研究表明，与 PIRVACoV 抗体研究和其他抗体 PFAS/COVID-19 研究一致，暴露于 PFAS 的健康成人对 mRNA COVID-19 疫苗接种可产生充分的免疫反应。EudraCT-number: 2021-000842-16.

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The T cell response to SARS-CoV-2 mRNA vaccine in adults with high exposure to perfluoroalkyl substances from Ronneby, Sweden

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The T cell response to SARS-CoV-2 mRNA vaccine in adults with high exposure to perfluoroalkyl substances from Ronneby, Sweden

Background

Perfluoroalkyl substances (PFAS) have been associated with impaired antibody levels after childhood vaccinations and immunosuppressive effects in animals. However, the in vivo effects of PFAS on antigen specific human T cell responses have not been investigated in adults. In Ronneby, Sweden, the drinking water of one of the water works was previously highly contaminated with primarily perfluorohexane sulfonic acid (PFHxS) and perfluorooctane sulfonic acid (PFOS). The COVID-19 vaccination scheme presented the possibility to assess antigen specific T cell function after vaccination in adults with high PFAS serum levels.

Objectives

To investigate the relationship between PFAS exposure and T cell responses after COVID-19 vaccination in a population with varied PFAS exposure.

Methods

116 COVID-19 naïve individuals from Ronneby and a background exposed group were included from the PFAS Immune Response After COVID-19 Vaccination cohort (PIRVACoV). All participants received two doses of Spikevax® (Moderna) vaccine. Blood T cells were stimulated with overlapping peptides based on the SARS-CoV-2 spike protein and their production of the cytokines IFN-γ, IL-2, and TNF were measured. The general immune response was assessed by measurement of phytohemagglutinin stimulated cytokines and total immunoglobulin serum levels. Adjusted mixed linear regressions were fitted against measured, address-based and prenatal PFAS exposure indices.

Results

PFAS median serum levels differed greatly between participants ever having had contaminated drinking water at home (PFOS 47 ng/mL, 5th to 95th percentile 6–221 ng/mL) and the background group (PFOS 4 ng/mL, 2–9 ng/mL). PFAS exposure was not associated with SARS-CoV-2 specific T cell cytokine responses (e.g., measured PFOS to IFN-γ: +3% per interquartile range PFOS, 95% confidence interval: 10, 17), nor general immune response.

Conclusions

This study indicates, in concordance with the PIRVACoV antibody study and other antibody PFAS/COVID-19 studies, that PFAS exposed, healthy adults mount adequate immune responses to mRNA COVID-19 vaccination. EudraCT-number: 2021-000842-16.

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来源期刊

Chemosphere 环境科学-环境科学

CiteScore

15.80

自引率

8.00%

发文量

4975

审稿时长

3.4 months

期刊介绍： Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.