蛋白磷酸酶 1 催化亚基 gamma 是导致肉质发亮和发红的致病基因。

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
Jiahong Sun, Xinting Yang, Guiping Zhao, Zhengxiao He, Wenhao Xing, Yanru Chen, Xiaodong Tan, Mengjie Wang, Wei Li, Bingxing An, Zhangyuan Pan, Zhengkui Zhou, Jie Wen, Ranran Liu
{"title":"蛋白磷酸酶 1 催化亚基 gamma 是导致肉质发亮和发红的致病基因。","authors":"Jiahong Sun, Xinting Yang, Guiping Zhao, Zhengxiao He, Wenhao Xing, Yanru Chen, Xiaodong Tan, Mengjie Wang, Wei Li, Bingxing An, Zhangyuan Pan, Zhengkui Zhou, Jie Wen, Ranran Liu","doi":"10.1371/journal.pgen.1011467","DOIUrl":null,"url":null,"abstract":"<p><p>The quality of meat is important to the consumer. Color is a primary indicator of meat quality and is characterized mainly into lightness, redness, and yellowness. Here, we used the genome-wide association study (GWAS) and gene-based association analysis with whole-genome resequencing of 230 fast-growing white-feathered chickens to map genes related to meat lightness and redness to a 6.24 kb QTL region (Chr15: 6298.34-6304.58 kb). This analysis revealed that only the protein phosphatase 1 catalytic subunit gamma (PPP1CC) was associated with meat color (P = 8.65E-08). The causal relationships between PPP1CC expression and meat lightness/redness were further validated through Mendelian randomization analyses (P < 2.9E-12). Inducible skeletal muscle-specific PPP1CC knockout (PPP1CC-SSKO) mice were generated and these mice showed increased lightness and decreased myoglobin content in the limb muscles. In addition, the predominant myofiber shifted from slow-twitch to fast-twitch myofibers. Through transcriptome and targeted metabolome evidence, we found that inhibition of PPP1CC decreased the expression of typical slow-twitch myofiber and myofiber-type specification genes and enhanced the glycolysis pathway. Functional validation through a plasmid reporter assay revealed that a SNP (rs315520807, C > T) located in the intron of PPP1CC could regulate the gene transcription activity. The differences in meat color phenotypes, myoglobin content, frequency of rs315520807 variant, expression of PPP1CC and fast-twitch fiber marker genes were detected between fast-growing white-feathered chickens and local chickens. In this study, PPP1CC was identified as the causative gene for meat color, and the novel target gene and variant that can aid in the innovation of meat improvement technology were detected.</p>","PeriodicalId":49007,"journal":{"name":"PLoS Genetics","volume":"20 11","pages":"e1011467"},"PeriodicalIF":4.0000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protein phosphatase 1 catalytic subunit gamma is a causative gene for meat lightness and redness.\",\"authors\":\"Jiahong Sun, Xinting Yang, Guiping Zhao, Zhengxiao He, Wenhao Xing, Yanru Chen, Xiaodong Tan, Mengjie Wang, Wei Li, Bingxing An, Zhangyuan Pan, Zhengkui Zhou, Jie Wen, Ranran Liu\",\"doi\":\"10.1371/journal.pgen.1011467\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The quality of meat is important to the consumer. Color is a primary indicator of meat quality and is characterized mainly into lightness, redness, and yellowness. Here, we used the genome-wide association study (GWAS) and gene-based association analysis with whole-genome resequencing of 230 fast-growing white-feathered chickens to map genes related to meat lightness and redness to a 6.24 kb QTL region (Chr15: 6298.34-6304.58 kb). This analysis revealed that only the protein phosphatase 1 catalytic subunit gamma (PPP1CC) was associated with meat color (P = 8.65E-08). The causal relationships between PPP1CC expression and meat lightness/redness were further validated through Mendelian randomization analyses (P < 2.9E-12). Inducible skeletal muscle-specific PPP1CC knockout (PPP1CC-SSKO) mice were generated and these mice showed increased lightness and decreased myoglobin content in the limb muscles. In addition, the predominant myofiber shifted from slow-twitch to fast-twitch myofibers. Through transcriptome and targeted metabolome evidence, we found that inhibition of PPP1CC decreased the expression of typical slow-twitch myofiber and myofiber-type specification genes and enhanced the glycolysis pathway. Functional validation through a plasmid reporter assay revealed that a SNP (rs315520807, C > T) located in the intron of PPP1CC could regulate the gene transcription activity. The differences in meat color phenotypes, myoglobin content, frequency of rs315520807 variant, expression of PPP1CC and fast-twitch fiber marker genes were detected between fast-growing white-feathered chickens and local chickens. In this study, PPP1CC was identified as the causative gene for meat color, and the novel target gene and variant that can aid in the innovation of meat improvement technology were detected.</p>\",\"PeriodicalId\":49007,\"journal\":{\"name\":\"PLoS Genetics\",\"volume\":\"20 11\",\"pages\":\"e1011467\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PLoS Genetics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1371/journal.pgen.1011467\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PLoS Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1371/journal.pgen.1011467","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

摘要

肉的质量对消费者来说非常重要。色泽是衡量肉质的主要指标,主要分为浅色、红色和黄色。在这里,我们利用全基因组关联研究(GWAS)和基于基因的关联分析,对230只快速生长的白羽鸡进行了全基因组重测序,将与肉的亮度和红度有关的基因映射到一个6.24 kb的QTL区域(Chr15: 6298.34-6304.58 kb)。分析表明,只有蛋白磷酸酶 1 催化亚基 gamma(PPP1CC)与肉色相关(P = 8.65E-08)。通过孟德尔随机分析(P < 2.9E-12),进一步验证了 PPP1CC 表达与肉的亮度/红度之间的因果关系。诱导性骨骼肌特异性 PPP1CC 基因敲除(PPP1CC-SSKO)小鼠产生,这些小鼠的四肢肌肉亮度增加,肌红蛋白含量降低。此外,主要的肌纤维也从慢速肌纤维转变为快速肌纤维。通过转录组和靶向代谢组证据,我们发现抑制 PPP1CC 会降低典型慢速肌纤维和肌纤维类型规格基因的表达,并增强糖酵解途径。通过质粒报告实验进行的功能验证发现,位于PPP1CC内含子的一个SNP(rs315520807,C > T)可调控该基因的转录活性。研究发现,速生白羽鸡与本地鸡在肉色表型、肌红蛋白含量、rs315520807变异频率、PPP1CC和快肌纤维标记基因的表达等方面存在差异。该研究确定了 PPP1CC 是肉色的致病基因,并发现了有助于肉色改良技术创新的新目标基因和变异体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protein phosphatase 1 catalytic subunit gamma is a causative gene for meat lightness and redness.

The quality of meat is important to the consumer. Color is a primary indicator of meat quality and is characterized mainly into lightness, redness, and yellowness. Here, we used the genome-wide association study (GWAS) and gene-based association analysis with whole-genome resequencing of 230 fast-growing white-feathered chickens to map genes related to meat lightness and redness to a 6.24 kb QTL region (Chr15: 6298.34-6304.58 kb). This analysis revealed that only the protein phosphatase 1 catalytic subunit gamma (PPP1CC) was associated with meat color (P = 8.65E-08). The causal relationships between PPP1CC expression and meat lightness/redness were further validated through Mendelian randomization analyses (P < 2.9E-12). Inducible skeletal muscle-specific PPP1CC knockout (PPP1CC-SSKO) mice were generated and these mice showed increased lightness and decreased myoglobin content in the limb muscles. In addition, the predominant myofiber shifted from slow-twitch to fast-twitch myofibers. Through transcriptome and targeted metabolome evidence, we found that inhibition of PPP1CC decreased the expression of typical slow-twitch myofiber and myofiber-type specification genes and enhanced the glycolysis pathway. Functional validation through a plasmid reporter assay revealed that a SNP (rs315520807, C > T) located in the intron of PPP1CC could regulate the gene transcription activity. The differences in meat color phenotypes, myoglobin content, frequency of rs315520807 variant, expression of PPP1CC and fast-twitch fiber marker genes were detected between fast-growing white-feathered chickens and local chickens. In this study, PPP1CC was identified as the causative gene for meat color, and the novel target gene and variant that can aid in the innovation of meat improvement technology were detected.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信