{"title":"高粱/高粱-苏丹草杂交种对肾形轮虫的抗病作用。","authors":"Roshan Paudel, Landon Balkwill, Koon-Hui Wang","doi":"10.1094/PDIS-08-24-1668-RE","DOIUrl":null,"url":null,"abstract":"<p><p>Reniform nematode (Rotylenchulus reniformis) is a damaging and difficult-to-manage pest on many agricultural crops. Sorghum and sorghum-sudangrass hybrids (SSgH) have shown potential in managing plant-parasitic nematodes by releasing toxic hydrogen cyanide gas through the hydrolysis of dhurrin, a cyanogenic glucoside found in leaf epidermal cells. The objectives of this study were to evaluate the effect of different SSgH varieties and their age on the suppression of R. reniformis and to quantify their dhurrin contents. Shoot biomass of seven SSgH varieties was harvested at 1, 2, and 3 months of growth and used as a soil amendment in two greenhouse cowpea bioassay trials. Dhurrin concentration was analyzed using high-performance liquid chromatography (HPLC) from 2-month-old tissues. The results indicated that energy sorghum 'NX-D-61' and SSgH 'Latte' exhibited the highest dhurrin concentrations (P ≤ 0.05) and suppressed R. reniformis development in cowpea roots (P ≤ 0.05). In Trial I, 2-month-old amendments showed the greatest suppression, while in Trial II, 1-month-old amendments were more suppressive (P ≤ 0.05). Potential effect of environmental stress on dhurrin concentration in SSgH tissue was discussed. Nonetheless, dhurrin concentrations were negatively related to the number of R. reniformis infecting cowpea roots (r2 =0.69; P = 0.02). These findings suggest that high dhurrin SSgH varieties can be integrated into reniform nematode management plans as a cover crop and terminated no more than 2 months after planting.</p>","PeriodicalId":20063,"journal":{"name":"Plant disease","volume":" ","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Allelopathic effects of sorghum/sorghum-sudangrass hybrids against <i>Rotylenchulus reniformis</i>.\",\"authors\":\"Roshan Paudel, Landon Balkwill, Koon-Hui Wang\",\"doi\":\"10.1094/PDIS-08-24-1668-RE\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Reniform nematode (Rotylenchulus reniformis) is a damaging and difficult-to-manage pest on many agricultural crops. Sorghum and sorghum-sudangrass hybrids (SSgH) have shown potential in managing plant-parasitic nematodes by releasing toxic hydrogen cyanide gas through the hydrolysis of dhurrin, a cyanogenic glucoside found in leaf epidermal cells. The objectives of this study were to evaluate the effect of different SSgH varieties and their age on the suppression of R. reniformis and to quantify their dhurrin contents. Shoot biomass of seven SSgH varieties was harvested at 1, 2, and 3 months of growth and used as a soil amendment in two greenhouse cowpea bioassay trials. Dhurrin concentration was analyzed using high-performance liquid chromatography (HPLC) from 2-month-old tissues. The results indicated that energy sorghum 'NX-D-61' and SSgH 'Latte' exhibited the highest dhurrin concentrations (P ≤ 0.05) and suppressed R. reniformis development in cowpea roots (P ≤ 0.05). In Trial I, 2-month-old amendments showed the greatest suppression, while in Trial II, 1-month-old amendments were more suppressive (P ≤ 0.05). Potential effect of environmental stress on dhurrin concentration in SSgH tissue was discussed. Nonetheless, dhurrin concentrations were negatively related to the number of R. reniformis infecting cowpea roots (r2 =0.69; P = 0.02). These findings suggest that high dhurrin SSgH varieties can be integrated into reniform nematode management plans as a cover crop and terminated no more than 2 months after planting.</p>\",\"PeriodicalId\":20063,\"journal\":{\"name\":\"Plant disease\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant disease\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PDIS-08-24-1668-RE\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PDIS-08-24-1668-RE","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
肾形线虫(Rotylenchulus reniformis)是一种对许多农作物具有破坏性且难以治理的害虫。高粱和高粱-苏丹草杂交种(SSgH)通过水解叶片表皮细胞中的氰基葡糖苷(dhurrin)释放出有毒的氰化氢气体,显示出治理植物寄生线虫的潜力。本研究的目的是评估不同 SSgH 品种及其年龄对抑制 R. reniformis 的影响,并量化它们的 dhurrin 含量。七个 SSgH 品种的嫩枝生物量分别在生长 1、2 和 3 个月时收获,并在两个温室豇豆生物测定试验中用作土壤改良剂。采用高效液相色谱法(HPLC)分析了生长 2 个月的高粱组织中 Dhurrin 的浓度。结果表明,能量高粱 "NX-D-61 "和 SSgH "Latte "表现出最高的 Dhurrin 浓度(P ≤ 0.05),并能抑制豇豆根部 R. reniformis 的生长(P ≤ 0.05)。在试验 I 中,使用 2 个月的改良剂的抑制作用最强,而在试验 II 中,使用 1 个月的改良剂的抑制作用更强(P ≤ 0.05)。讨论了环境胁迫对 SSgH 组织中 dhurrin 浓度的潜在影响。然而,dhurrin 浓度与感染豇豆根部的 R. reniformis 数量呈负相关(r2 =0.69; P = 0.02)。这些研究结果表明,高白藜芦醇 SSgH 品种可作为覆盖作物纳入肾形线虫管理计划,并在种植后 2 个月内终止。
Allelopathic effects of sorghum/sorghum-sudangrass hybrids against Rotylenchulus reniformis.
Reniform nematode (Rotylenchulus reniformis) is a damaging and difficult-to-manage pest on many agricultural crops. Sorghum and sorghum-sudangrass hybrids (SSgH) have shown potential in managing plant-parasitic nematodes by releasing toxic hydrogen cyanide gas through the hydrolysis of dhurrin, a cyanogenic glucoside found in leaf epidermal cells. The objectives of this study were to evaluate the effect of different SSgH varieties and their age on the suppression of R. reniformis and to quantify their dhurrin contents. Shoot biomass of seven SSgH varieties was harvested at 1, 2, and 3 months of growth and used as a soil amendment in two greenhouse cowpea bioassay trials. Dhurrin concentration was analyzed using high-performance liquid chromatography (HPLC) from 2-month-old tissues. The results indicated that energy sorghum 'NX-D-61' and SSgH 'Latte' exhibited the highest dhurrin concentrations (P ≤ 0.05) and suppressed R. reniformis development in cowpea roots (P ≤ 0.05). In Trial I, 2-month-old amendments showed the greatest suppression, while in Trial II, 1-month-old amendments were more suppressive (P ≤ 0.05). Potential effect of environmental stress on dhurrin concentration in SSgH tissue was discussed. Nonetheless, dhurrin concentrations were negatively related to the number of R. reniformis infecting cowpea roots (r2 =0.69; P = 0.02). These findings suggest that high dhurrin SSgH varieties can be integrated into reniform nematode management plans as a cover crop and terminated no more than 2 months after planting.
期刊介绍:
Plant Disease is the leading international journal for rapid reporting of research on new, emerging, and established plant diseases. The journal publishes papers that describe basic and applied research focusing on practical aspects of disease diagnosis, development, and management.