评估直接淋巴细胞增殖试验,以诊断口服食物后出现延迟反应的犬类食物过敏症。

IF 1.9 3区 农林科学 Q3 DERMATOLOGY
Carlos Fernandez-Lozano, Ana Mas-Fontao, Silvia T Auxilia, Marie Welters, Alla Olivri, Ralf S Mueller, Thierry Olivry
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引用次数: 0

摘要

背景:在人类中,食物过敏(FAs)可分为免疫球蛋白(Ig)E介导的过敏(即刻过敏)、细胞介导的过敏(延迟过敏)或两种机制同时存在的过敏(混合过敏)。在犬中,淋巴细胞刺激试验与口服食物挑战(OFC)的一致性最高:目的:报告淋巴细胞增殖试验(LPT)在口服食物挑战(OFC)后出现FA和延迟反应(≥6小时)的犬中的评估结果:动物:35只健康犬和28只延迟FA犬:分离外周血单核细胞(PBMC),并在与食物过敏原进行为期 5 天的培养前后自动计数。然后计算刺激指数(SI)。使用宠物过敏 Xplorer (PAX) 对食物过敏原特异性 IgE 进行量化:结果:在 10 只健康的实验用小猎犬和 25 只健康的宠物狗中,没有一只的刺激指数大于 3.0 临界值,这表明特异性为 100%。所有 28 只患有延迟性 FA 的狗在 OFC 后至少有一次对诱发延迟性发作的食物项目的刺激呈阳性;该 LPT 鉴定延迟性犬 FA 的灵敏度为 100%。LPT 能正确识别 68 种食物中的 57 种(84%),这些食物会在 OFC 后引起延迟性发烧。在 28 只狗中,有 18 只狗(64%)的 PAX 检测结果为食物特异性 IgE 阴性,这也是延迟性 FA 的预期结果。有三只狗(11%)的 PAX 结果与 LPT 结果重叠,这表明它们是混合型 FA:结论和临床意义:OFC 后出现延迟反应的食物过敏--那些被怀疑具有细胞介导机制的食物过敏--似乎是研究犬中最常见的 FA 类型。LPT有助于识别此类病例。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of a direct lymphocyte proliferation test for the diagnosis of canine food allergies with delayed reactions after oral food challenge.

Background: In humans, food allergies (FAs) are divided into those with immunoglobulin (Ig) E-mediated (immediate FA), cell-mediated (delayed FA) or both mechanisms (mixed FA). In dogs, lymphocyte stimulation tests have the highest concordance with oral food challenges (OFCs).

Objectives: To report the evaluation of a lymphocyte proliferation test (LPT) in dogs with FA and delayed reactions (≥6 h) after OFC.

Animals: Thirty-five healthy and 28 dogs with delayed FA.

Materials and methods: Peripheral blood mononuclear cells (PBMCs) were isolated and automatically counted before and after a 5-day culture with food allergens. Stimulation indices (SIs) were then calculated. Food allergen-specific IgE was quantified using the Pet Allergy Xplorer (PAX).

Results: None of the 10 healthy laboratory beagles and 25 healthy pet dogs had an SI greater than the 3.0 cut-off, indicating a specificity of 100%. All 28 dogs with delayed FA had at least one positive stimulation to a food item that induced delayed flares after OFC; the sensitivity of this LPT for the identification of delayed canine FA was 100%. The LPT correctly identified 57 of 68 food items causing delayed flares after OFC (84%). The PAX was negative for food-specific IgE in 18 of the 28 dogs (64%), as expected for delayed FA. In three dogs (11%), PAX results overlapped with those of the LPT, suggesting a mixed FA.

Conclusions and clinical relevance: Food allergies with delayed reactions after OFC-those suspected of having a cell-mediated mechanism-seemed to be the most common type of FA in the studied dogs. The LPT was helpful in identifying such cases.

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来源期刊
Veterinary dermatology
Veterinary dermatology 农林科学-兽医学
CiteScore
3.20
自引率
21.40%
发文量
92
审稿时长
12-24 weeks
期刊介绍: Veterinary Dermatology is a bi-monthly, peer-reviewed, international journal which publishes papers on all aspects of the skin of mammals, birds, reptiles, amphibians and fish. Scientific research papers, clinical case reports and reviews covering the following aspects of dermatology will be considered for publication: -Skin structure (anatomy, histology, ultrastructure) -Skin function (physiology, biochemistry, pharmacology, immunology, genetics) -Skin microbiology and parasitology -Dermatopathology -Pathogenesis, diagnosis and treatment of skin diseases -New disease entities
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