城市化和再生水厂对洛杉矶河流域粪便指示细菌和抗生素耐药性的影响:采用互补监测方法的案例研究。

IF 8.2 1区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Science of the Total Environment Pub Date : 2024-12-20 Epub Date: 2024-11-21 DOI:10.1016/j.scitotenv.2024.177577
Ileana A Callejas, Yuwei Kong, Katie Osborn, Wei-Cheng Hung, Marisol Cira, Taylor Cason, Ashlyn Sloane, Alexis Shenkiryk, Aaron Masikip, Akshyae Singh, Adriane Jones, Joshua A Steele, Jennifer A Jay
{"title":"城市化和再生水厂对洛杉矶河流域粪便指示细菌和抗生素耐药性的影响:采用互补监测方法的案例研究。","authors":"Ileana A Callejas, Yuwei Kong, Katie Osborn, Wei-Cheng Hung, Marisol Cira, Taylor Cason, Ashlyn Sloane, Alexis Shenkiryk, Aaron Masikip, Akshyae Singh, Adriane Jones, Joshua A Steele, Jennifer A Jay","doi":"10.1016/j.scitotenv.2024.177577","DOIUrl":null,"url":null,"abstract":"<p><p>Urban land use and water reclamation plants (WRPs) can impact fecal indicator bacteria (FIB) and antimicrobial resistance (AMR) in coastal watersheds. However, there is a lack of studies exploring these effects on the US West Coast. Additionally, there is limited research using a complementary approach across culture-, qPCR-, and metagenomics-based techniques for characterizing environmental AMR. In this study, sixteen locations were sampled in the Los Angeles River, encompassing both upstream and downstream of three WRPs discharging into the river. Culture-dependent methods quantified Enterococcus, total coliforms, E. coli, and extended spectrum beta-lactamase-producing E. coli as a low-cost screening tool for AMR, while qPCR measured selected antibiotic resistance genes (ARGs): sul1, ermF, tetW, blaSHV, along with intI1 and 16S rRNA genes. Bacteroides HF183 and crAssphage markers were quantified via ddPCR. All samples underwent shotgun sequencing to investigate gene abundance and mobility and an overall risk score for AMR. Results reveal downstream sites contain ARGs at least two orders of magnitude greater than upstream locations. Developed areas had the highest ARG sequence abundances and the most ARG classes as indicated by metagenomic analysis. WRP effluent exhibited elevated ARGs and co-location of ARGs, mobile genetic elements, and pathogens. A culture-based assessment of AR in E. coli and Pseudomonas aeruginosa revealed increased resistance ratios for most antibiotics from upstream to downstream a WRP discharge point. This study highlights the impacts of land use and WRPs on ARGs and FIB, offering a multi-pronged analysis of AMR.</p>","PeriodicalId":422,"journal":{"name":"Science of the Total Environment","volume":" ","pages":"177577"},"PeriodicalIF":8.2000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The influence of urbanization and water reclamation plants on fecal indicator bacteria and antibiotic resistance in the Los Angeles River watershed: A case study with complementary monitoring methods.\",\"authors\":\"Ileana A Callejas, Yuwei Kong, Katie Osborn, Wei-Cheng Hung, Marisol Cira, Taylor Cason, Ashlyn Sloane, Alexis Shenkiryk, Aaron Masikip, Akshyae Singh, Adriane Jones, Joshua A Steele, Jennifer A Jay\",\"doi\":\"10.1016/j.scitotenv.2024.177577\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Urban land use and water reclamation plants (WRPs) can impact fecal indicator bacteria (FIB) and antimicrobial resistance (AMR) in coastal watersheds. However, there is a lack of studies exploring these effects on the US West Coast. Additionally, there is limited research using a complementary approach across culture-, qPCR-, and metagenomics-based techniques for characterizing environmental AMR. In this study, sixteen locations were sampled in the Los Angeles River, encompassing both upstream and downstream of three WRPs discharging into the river. Culture-dependent methods quantified Enterococcus, total coliforms, E. coli, and extended spectrum beta-lactamase-producing E. coli as a low-cost screening tool for AMR, while qPCR measured selected antibiotic resistance genes (ARGs): sul1, ermF, tetW, blaSHV, along with intI1 and 16S rRNA genes. Bacteroides HF183 and crAssphage markers were quantified via ddPCR. All samples underwent shotgun sequencing to investigate gene abundance and mobility and an overall risk score for AMR. Results reveal downstream sites contain ARGs at least two orders of magnitude greater than upstream locations. Developed areas had the highest ARG sequence abundances and the most ARG classes as indicated by metagenomic analysis. WRP effluent exhibited elevated ARGs and co-location of ARGs, mobile genetic elements, and pathogens. A culture-based assessment of AR in E. coli and Pseudomonas aeruginosa revealed increased resistance ratios for most antibiotics from upstream to downstream a WRP discharge point. This study highlights the impacts of land use and WRPs on ARGs and FIB, offering a multi-pronged analysis of AMR.</p>\",\"PeriodicalId\":422,\"journal\":{\"name\":\"Science of the Total Environment\",\"volume\":\" \",\"pages\":\"177577\"},\"PeriodicalIF\":8.2000,\"publicationDate\":\"2024-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Science of the Total Environment\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1016/j.scitotenv.2024.177577\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/11/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science of the Total Environment","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.scitotenv.2024.177577","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/21 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

城市土地利用和水再生厂(WRPs)会影响沿海流域的粪便指示菌(FIB)和抗菌药耐药性(AMR)。然而,目前还缺乏对美国西海岸这些影响的研究。此外,使用基于培养、qPCR 和元基因组学技术的互补方法来描述环境 AMR 特征的研究也很有限。在这项研究中,对洛杉矶河的 16 个地点进行了采样,包括向该河排放污水的三个水处理厂的上游和下游。培养法量化了肠球菌、总大肠菌群、大肠杆菌和产广谱β-内酰胺酶大肠杆菌,作为一种低成本的 AMR 筛查工具,而 qPCR 则测量了选定的抗生素耐药基因 (ARGs):Sul1、ermF、tetW、blaSHV 以及 intI1 和 16S rRNA 基因。通过 ddPCR 对 HF183 菌和 crAssphage 标记进行了量化。所有样本都进行了霰弹枪测序,以调查基因丰度和流动性以及 AMR 的总体风险评分。结果显示,下游地点的 ARGs 含量至少比上游地点高两个数量级。元基因组分析表明,发达地区的 ARG 序列丰度最高,ARG 种类也最多。水污染防治计划(WRP)的污水中 ARGs 增高,ARGs、移动遗传因子和病原体共存。基于培养的大肠杆菌和铜绿假单胞菌抗药性评估显示,从水污染防治计划排放点的上游到下游,大多数抗生素的抗药性比率都在增加。这项研究强调了土地利用和水处理厂对 ARGs 和 FIB 的影响,对 AMR 进行了多管齐下的分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The influence of urbanization and water reclamation plants on fecal indicator bacteria and antibiotic resistance in the Los Angeles River watershed: A case study with complementary monitoring methods.

Urban land use and water reclamation plants (WRPs) can impact fecal indicator bacteria (FIB) and antimicrobial resistance (AMR) in coastal watersheds. However, there is a lack of studies exploring these effects on the US West Coast. Additionally, there is limited research using a complementary approach across culture-, qPCR-, and metagenomics-based techniques for characterizing environmental AMR. In this study, sixteen locations were sampled in the Los Angeles River, encompassing both upstream and downstream of three WRPs discharging into the river. Culture-dependent methods quantified Enterococcus, total coliforms, E. coli, and extended spectrum beta-lactamase-producing E. coli as a low-cost screening tool for AMR, while qPCR measured selected antibiotic resistance genes (ARGs): sul1, ermF, tetW, blaSHV, along with intI1 and 16S rRNA genes. Bacteroides HF183 and crAssphage markers were quantified via ddPCR. All samples underwent shotgun sequencing to investigate gene abundance and mobility and an overall risk score for AMR. Results reveal downstream sites contain ARGs at least two orders of magnitude greater than upstream locations. Developed areas had the highest ARG sequence abundances and the most ARG classes as indicated by metagenomic analysis. WRP effluent exhibited elevated ARGs and co-location of ARGs, mobile genetic elements, and pathogens. A culture-based assessment of AR in E. coli and Pseudomonas aeruginosa revealed increased resistance ratios for most antibiotics from upstream to downstream a WRP discharge point. This study highlights the impacts of land use and WRPs on ARGs and FIB, offering a multi-pronged analysis of AMR.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Science of the Total Environment
Science of the Total Environment 环境科学-环境科学
CiteScore
17.60
自引率
10.20%
发文量
8726
审稿时长
2.4 months
期刊介绍: The Science of the Total Environment is an international journal dedicated to scientific research on the environment and its interaction with humanity. It covers a wide range of disciplines and seeks to publish innovative, hypothesis-driven, and impactful research that explores the entire environment, including the atmosphere, lithosphere, hydrosphere, biosphere, and anthroposphere. The journal's updated Aims & Scope emphasizes the importance of interdisciplinary environmental research with broad impact. Priority is given to studies that advance fundamental understanding and explore the interconnectedness of multiple environmental spheres. Field studies are preferred, while laboratory experiments must demonstrate significant methodological advancements or mechanistic insights with direct relevance to the environment.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信