Contrasting environmental impacts of nano-biochar and conventional biochar on various organisms.

The environmental hazards of nanobiochar (n-BC) require attention due to limited knowledge. This study is the first to explore the effects of biochar size reduction across various organisms, including bacteria (Allivibrio fischeri), plants (Lemna minor, Lepidium sativum), and invertebrates (Daphnia magna, Folsomia candida). Bulk biochar (b-BC) and n-BC were applied in both liquid and solid-phase tests to assess their ecotoxicity. The resulting leachates were tested at concentrations of 2, 10, and 100 mg/L on organisms such as Daphnia magna and Lemna minor. In the solid-phase tests, b-BC and n-BC were added to the OECD soil at concentrations of 1 % and 5 % to evaluate toxicity in Folsomia candida and at concentrations of 1 % to evaluate toxicity in Lepidium sativum. We found n-BC to be significantly more toxic (by 18 % to 2886 %) to A. fischeri than b-BC, with toxicity increasing over time. Low doses (1 %) of both b-BC and n-BC did not cause mortality or inhibit reproduction in F. candida, though b-BC enhanced reproduction (by 30 % to 56 %) compared to n-BC. At a 5 % dose, both b-BC and n-BC inhibited reproduction F. candida, with n-BC being 0.5 to 1.8 times more toxic. Neither b-BC nor n-BC immobilized D. magna, but both inhibited reproduction (by 28 % to 35 %). The nanoscale dimensions of n-BC facilitated bioaccumulation in D. magna, leading to adhesion on the organism's body. The n-BC had a greater impact on plants, both b-BC and n-BC were non-toxic to L. minor, but all n-BC inhibited root growth in L. sativum. These findings highlight the importance of considering biochar size, feedstock, and pyrolysis conditions when evaluating environmental risks, ensuring safe use in sustainable agriculture.