PDCD6在胶质母细胞瘤干性维持中的作用

IF 2.9 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2024-11-16 DOI:10.1016/j.prp.2024.155727

Xiyu Liu , Zuolin Shi , Xuantong Liu , Yuan Cao , Xinyu Yang , Jiaming Liu , Tianqi Xu , Weiyi Yang , Ligang Chen , Zheng Zou , Qingge Jia , Mingyang Li

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引用次数: 0

摘要

背景：胶质母细胞瘤（GBM）因其高度恶性和治疗耐药性而面临严峻挑战，其 5 年生存率仍然很低、复发率高且治疗方法有限。由于缺乏成熟的治疗方案，复发性胶质母细胞瘤急需创新的治疗方法。这一需求推动了对引发和推动疾病发展的细胞基础的研究，旨在发现突破性的治疗靶点，从而提高医疗干预措施的疗效：方法：采集并分离源自患者的胶质母细胞瘤干细胞（GSCs）。随后，通过Western印迹（WB）和实时PCR（RT-PCR）技术对PDCD6的表达进行量化。利用球形成试验评估了 GSCs 的干样特性。所有收集到的数据（包括TCGA数据集）都使用SPSS（IBM）23.0版进行了分析：PDCD6的高表达是经典GBM肿瘤组织的特征。PDCD6 的过表达与 GBM 患者总生存期的缩短密切相关，是一个独立的预后指标。值得注意的是，与NSTCs相比，原代GBM细胞中GSCs的PDCD6水平更高。此外，干性标志物的改变与PDCD6的调节同步，PDCD6的敲除减小了GSCs的肿瘤大小：我们的研究结果阐明了PDCD6在经典GBM中培养干性的作用，暗示了其作为治疗靶点的潜力。

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The role of PDCD6 in stemness maintenance of Glioblastoma

Background

Glioblastoma (GBM) poses formidable challenges due to its high malignancy and therapeutic resistance and still exhibits dismal 5-year survival rates, high recurrence propensity, and limited treatment modalities. There is an acute need for innovative treatments for recurrent glioblastoma due to the lack of established protocols. This necessity is driving research into the cellular underpinnings that initiate and drive the disease forward, aiming to discover groundbreaking targets for therapy that could enhance the efficacy of medical interventions.

Methods

Patient-derived glioblastoma stem cells (GSCs) were harvested and isolated. Subsequently, PDCD6 expression was quantified through both western blotting (WB) and real-time PCR (RT-PCR) techniques. The stem-like properties of the GSCs were evaluated using sphere-forming assays. All gathered data, inclusive of TCGA datasets, were analyzed using SPSS (IBM) version 23.0.

Results

Elevated PDCD6 expression characterized classical GBM tumor tissues. PDCD6 overexpression significantly correlated with diminished overall survival in GBM patients, emerging as an independent prognostic indicator. Notably, primary GBM cells exhibited heightened PDCD6 levels in GSCs compared to NSTCs. Moreover, alterations in stemness markers paralleled PDCD6 modulation, where PDCD6 knockdown attenuated tumor size in GSCs.

Conclusion

Our findings illuminate PDCD6's role in fostering stemness within classical GBM, hinting at its potential as a therapeutic target.

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.