Da Hee Yang, Hyo Jin Kim, Duong Thi Thuy Dinh, Jiwon Yang, Chang-Lim Hyun, Youngheun Jee, Naeun Lee, Min Sun Shin, Insoo Kang, Ki Soo Kang
{"title":"炎症性肠病患儿中与粘膜免疫相关的 IL-7RαlowCX3CR1+ CD8+ T 细胞和 α4β7 Integrin 标记 T 细胞的表达。","authors":"Da Hee Yang, Hyo Jin Kim, Duong Thi Thuy Dinh, Jiwon Yang, Chang-Lim Hyun, Youngheun Jee, Naeun Lee, Min Sun Shin, Insoo Kang, Ki Soo Kang","doi":"10.5223/pghn.2024.27.6.345","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The study aimed to investigate the recruiting of T lymphocytes including IL-7Rα<sup>low</sup> CX3CR1<sup>+</sup> effector memory (EM) CD8<sup>+</sup> T cells and α4β7 integrin tagged T cells to inflamed intestinal mucosa.</p><p><strong>Methods: </strong>Whole blood and mucosal tissues of intestine were collected from 40 children with or without inflammatory bowel disease (IBD). T cell surface staining and immunohistochemistry were done with several antibodies in peripheral blood mononuclear cells (PBMCs) and intestinal mucosa, respectively. Serum levels of cytokines were measured by ELISA.</p><p><strong>Results: </strong>The frequency of IL-7Rα<sup>low</sup>CX3CR1<sup>+</sup> EM CD8<sup>+</sup> T cells in the PBMC was significantly higher in the ulcerative colitis group than in the control group (57.9±17.80% vs. 33.9±15.70%, <i>p</i>=0.021). The frequency of integrin α4β7<sup>+</sup> CD4<sup>+</sup> T cells in the PBMC was significantly lower in the ulcerative colitis group than in the control group (53.2±27.6% vs. 63.9±13.2%, <i>p</i>=0.022). Serum concentration of TNF-α was higher in the Crohn's disease group than in the control group (26.13±5.01 pg/mL vs. 19.65±6.07 pg/mL, <i>p</i>=0.008). Of the three groups, the ulcerative colitis group had the highest frequency of integrin α4β7<sup>+</sup> T cells based on immunohistochemistry analyses for intestinal tissues, followed by the Crohn's disease group and the control group (4.63±1.29 cells vs. 2.0±0.57 cells vs. 0.84±0.52 cells, <i>p</i><0.001).</p><p><strong>Conclusion: </strong>Trafficking immune cells with effector memory CD8<sup>+</sup> T cells clarified by IL-7Rα<sup>low</sup>CX3CR1<sup>+</sup> and integrin α4β7<sup>+</sup> CD4<sup>+</sup> T cells might be highly associated with the pathogenesis of ulcerative colitis.</p>","PeriodicalId":19989,"journal":{"name":"Pediatric Gastroenterology, Hepatology & Nutrition","volume":"27 6","pages":"345-354"},"PeriodicalIF":1.3000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11570351/pdf/","citationCount":"0","resultStr":"{\"title\":\"Expression of IL-7Rα<sup>low</sup>CX3CR1<sup>+</sup> CD8<sup>+</sup> T Cells and α4β7 Integrin Tagged T Cells Related to Mucosal Immunity in Children with Inflammatory Bowel Disease.\",\"authors\":\"Da Hee Yang, Hyo Jin Kim, Duong Thi Thuy Dinh, Jiwon Yang, Chang-Lim Hyun, Youngheun Jee, Naeun Lee, Min Sun Shin, Insoo Kang, Ki Soo Kang\",\"doi\":\"10.5223/pghn.2024.27.6.345\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>The study aimed to investigate the recruiting of T lymphocytes including IL-7Rα<sup>low</sup> CX3CR1<sup>+</sup> effector memory (EM) CD8<sup>+</sup> T cells and α4β7 integrin tagged T cells to inflamed intestinal mucosa.</p><p><strong>Methods: </strong>Whole blood and mucosal tissues of intestine were collected from 40 children with or without inflammatory bowel disease (IBD). T cell surface staining and immunohistochemistry were done with several antibodies in peripheral blood mononuclear cells (PBMCs) and intestinal mucosa, respectively. Serum levels of cytokines were measured by ELISA.</p><p><strong>Results: </strong>The frequency of IL-7Rα<sup>low</sup>CX3CR1<sup>+</sup> EM CD8<sup>+</sup> T cells in the PBMC was significantly higher in the ulcerative colitis group than in the control group (57.9±17.80% vs. 33.9±15.70%, <i>p</i>=0.021). The frequency of integrin α4β7<sup>+</sup> CD4<sup>+</sup> T cells in the PBMC was significantly lower in the ulcerative colitis group than in the control group (53.2±27.6% vs. 63.9±13.2%, <i>p</i>=0.022). Serum concentration of TNF-α was higher in the Crohn's disease group than in the control group (26.13±5.01 pg/mL vs. 19.65±6.07 pg/mL, <i>p</i>=0.008). Of the three groups, the ulcerative colitis group had the highest frequency of integrin α4β7<sup>+</sup> T cells based on immunohistochemistry analyses for intestinal tissues, followed by the Crohn's disease group and the control group (4.63±1.29 cells vs. 2.0±0.57 cells vs. 0.84±0.52 cells, <i>p</i><0.001).</p><p><strong>Conclusion: </strong>Trafficking immune cells with effector memory CD8<sup>+</sup> T cells clarified by IL-7Rα<sup>low</sup>CX3CR1<sup>+</sup> and integrin α4β7<sup>+</sup> CD4<sup>+</sup> T cells might be highly associated with the pathogenesis of ulcerative colitis.</p>\",\"PeriodicalId\":19989,\"journal\":{\"name\":\"Pediatric Gastroenterology, Hepatology & Nutrition\",\"volume\":\"27 6\",\"pages\":\"345-354\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11570351/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pediatric Gastroenterology, Hepatology & Nutrition\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5223/pghn.2024.27.6.345\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/11/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"PEDIATRICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pediatric Gastroenterology, Hepatology & Nutrition","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5223/pghn.2024.27.6.345","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/5 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"PEDIATRICS","Score":null,"Total":0}
引用次数: 0
摘要
目的：该研究旨在调查T淋巴细胞（包括IL-7Rα-low CX3CR1+效应记忆（EM）CD8+T细胞和α4β7整合素标记T细胞）对炎症肠粘膜的招募情况：方法：收集 40 名患有或未患有炎症性肠病（IBD）的儿童的全血和肠道粘膜组织。用几种抗体分别对外周血单核细胞（PBMCs）和肠粘膜进行了 T 细胞表面染色和免疫组化。用酶联免疫吸附法测定了血清中细胞因子的水平：结果：溃疡性结肠炎组 PBMC 中 IL-7RαlowCX3CR1+ EM CD8+ T 细胞的频率明显高于对照组（57.9±17.80% vs. 33.9±15.70%，P=0.021）。溃疡性结肠炎组 PBMC 中整合素 α4β7+ CD4+ T 细胞的频率明显低于对照组（53.2±27.6% vs. 63.9±13.2%，P=0.022）。克罗恩病组的血清 TNF-α 浓度高于对照组（26.13±5.01 pg/mL vs. 19.65±6.07 pg/mL，P=0.008）。根据肠道组织的免疫组化分析，三组中溃疡性结肠炎组的整合素α4β7+ T细胞频率最高，其次是克罗恩病组和对照组（4.63±1.29 cells vs. 2.0±0.57 cells vs. 0.84±0.52 cells，P=0.008）：通过IL-7Rα-lowCX3CR1+和整合素α4β7+ CD4+ T细胞明确的具有效应记忆CD8+ T细胞的交通免疫细胞可能与溃疡性结肠炎的发病机制高度相关。
Expression of IL-7RαlowCX3CR1+ CD8+ T Cells and α4β7 Integrin Tagged T Cells Related to Mucosal Immunity in Children with Inflammatory Bowel Disease.
Purpose: The study aimed to investigate the recruiting of T lymphocytes including IL-7Rαlow CX3CR1+ effector memory (EM) CD8+ T cells and α4β7 integrin tagged T cells to inflamed intestinal mucosa.
Methods: Whole blood and mucosal tissues of intestine were collected from 40 children with or without inflammatory bowel disease (IBD). T cell surface staining and immunohistochemistry were done with several antibodies in peripheral blood mononuclear cells (PBMCs) and intestinal mucosa, respectively. Serum levels of cytokines were measured by ELISA.
Results: The frequency of IL-7RαlowCX3CR1+ EM CD8+ T cells in the PBMC was significantly higher in the ulcerative colitis group than in the control group (57.9±17.80% vs. 33.9±15.70%, p=0.021). The frequency of integrin α4β7+ CD4+ T cells in the PBMC was significantly lower in the ulcerative colitis group than in the control group (53.2±27.6% vs. 63.9±13.2%, p=0.022). Serum concentration of TNF-α was higher in the Crohn's disease group than in the control group (26.13±5.01 pg/mL vs. 19.65±6.07 pg/mL, p=0.008). Of the three groups, the ulcerative colitis group had the highest frequency of integrin α4β7+ T cells based on immunohistochemistry analyses for intestinal tissues, followed by the Crohn's disease group and the control group (4.63±1.29 cells vs. 2.0±0.57 cells vs. 0.84±0.52 cells, p<0.001).
Conclusion: Trafficking immune cells with effector memory CD8+ T cells clarified by IL-7RαlowCX3CR1+ and integrin α4β7+ CD4+ T cells might be highly associated with the pathogenesis of ulcerative colitis.
期刊介绍：
Pediatric Gastroenterology, Hepatology and Nutrition (Pediatr Gastroenterol Hepatol Nutr), an official journal of The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition, is issued bimonthly and published in English. The aim of Pediatr Gastroenterol Hepatol Nutr is to advance scientific knowledge and promote child healthcare by publishing high-quality empirical and theoretical studies and providing a recently updated knowledge to those practitioners and scholars in the field of pediatric gastroenterology, hepatology and nutrition. Pediatr Gastroenterol Hepatol Nutr publishes review articles, original articles, and case reports. All of the submitted papers are peer-reviewed. The journal covers basic and clinical researches on molecular and cellular biology, pathophysiology, epidemiology, diagnosis, and treatment of all aspects of pediatric gastrointestinal diseases and nutritional health problems.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
