白藜芦醇通过 Nrf2 途径抑制中暑诱导的大鼠肺组织中的铁蛋白沉积。

IF 2.8 3区 医学 Q2 PHARMACOLOGY & PHARMACY
Liwen Du, Xueqi Zhu, Zhenluo Jiang, Weidong Wang, Peng Liu, Leilei Zhu, Fangqi Zhang
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引用次数: 0

摘要

背景:中暑(HS)可导致肺铁沉着病的发生。在 HS 期间抑制肺铁蛋白沉积可改善患者的预后。本研究旨在探讨白藜芦醇(RES)对环境温度为 42 ℃ 的热应激的影响。通过尾静脉注射核因子-2相关因子(Nrf2)shRNA重组腺相关病毒6(AAV6-shNrf2)证实了RES的抗氧化应激和抗发炎作用:结果:RES治疗减轻了活性氧(ROS)和丙二醛(MDA)水平的上调,并缓解了谷胱甘肽在HS中的抑制作用。此外,RES还能减少热应激Beas-2B细胞中Fe2+的积累,增加铁变态反应抵抗相关蛋白FTH1、GPX4和SLC7A11以及抗氧化应激通路蛋白Nrf2、NQO1和HO-1。用Nrf2通路抑制剂Nrf2-IN-1处理热应激Beas-2B细胞后,RES的抗氧化和抗铁锈作用被有效逆转。在 HS 大鼠模型中,环境温度为 42 °C,相对湿度为 60 ± 5%时,RES 的抗氧化和抗发炎作用被逆转:结论:RES 能有效保护 HS 大鼠免受肺损伤,抑制肺内 Fe2+、ROS 和 MDA 的积累,并上调 FTH1、GPX4、SLC7A11、Nrf2、NQO1 和 HO-1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Resveratrol inhibits ferroptosis in the lung tissues of heat stroke-induced rats via the Nrf2 pathway.

Background: Heat stroke (HS) can lead to the development of pulmonary ferroptosis. The inhibition of pulmonary ferroptosis during HS improves patient prognosis. The aim of this study was to investigate the effects of resveratrol (RES) on heat stress at an ambient temperature of 42 °C.

Methods: Heat stress was induced in Beas-2B cells and lung injury was induced in HS rats at an ambient temperature of 42 °C. The anti-oxidative stress and anti-ferroptotic effects of RES were confirmed through tail vein injection of nuclear factor-2 associated factor (Nrf2) shRNA recombinant adeno-associated virus 6 (AAV6-shNrf2).

Results: RES treatment attenuated the upregulation of reactive oxygen species (ROS) and malondialdehyde (MDA) levels and alleviated glutathione inhibition in HS. In addition, RES treatment reduced the accumulation of Fe2+ in heat-stressed Beas-2B cells and increased the ferroptosis resistance-related proteins FTH1, GPX4, and SLC7A11 as well as the anti-oxidative stress pathway proteins Nrf2, NQO1, and HO-1. The antioxidant and anti-ferroptotic effects of RES in heat-stressed Beas-2B cells were effectively reversed upon treatment with Nrf2-IN-1, an Nrf2 pathway inhibitor. In the HS rat model, the antioxidant and anti-ferroptotic effects of RES were reversed by an ambient temperature of 42 °C and relative humidity of 60 ± 5%.

Conclusions: RES effectively protected HS rats from lung injury, inhibited the accumulation of Fe2+, ROS, and MDA in the lung, and upregulated FTH1, GPX4, SLC7A11, Nrf2, NQO1, and HO-1.

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来源期刊
BMC Pharmacology & Toxicology
BMC Pharmacology & Toxicology PHARMACOLOGY & PHARMACYTOXICOLOGY&nb-TOXICOLOGY
CiteScore
4.80
自引率
0.00%
发文量
87
审稿时长
12 weeks
期刊介绍: BMC Pharmacology and Toxicology is an open access, peer-reviewed journal that considers articles on all aspects of chemically defined therapeutic and toxic agents. The journal welcomes submissions from all fields of experimental and clinical pharmacology including clinical trials and toxicology.
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