室温定量液体浓缩装置及其在 B 细胞培养基中白细胞介素分析中的应用。

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL
Ruriko Kawanabe, Hidekatsu Tazawa, Kazuma Mawatari, Ayumi Yoshizaki
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引用次数: 0

摘要

在生物分析和医学诊断中,人们越来越需要在不降低定量性的前提下提高检测下限,但这通常具有挑战性。在这项研究中,我们利用旋流装置建立了一种液体浓缩方法。空气旋流在浓缩装置中形成液体旋流,增强了空气/液体界面面积,从而在室温下实现了从毫升到毫升的液体有效浓缩。研究了加热温度和液体内部的实际温度,以了解蒸发的冷却效果。标准溶液的收集效率大于 98%。最后,建立了实现定量浓缩的分析程序,并演示了从 B 细胞培养基上清液中浓缩和定量白细胞介素(IL-8、IL-17 和 IL-23)。用 CD40L 刺激 B 细胞,上清液最大浓缩 27 倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Room temperature quantitative liquid concentration device and application to interleukins analysis in a B-cell culture medium.

In biological analysis and medical diagnosis, there is an increasing demand for improving the lower detection limit without deteriorating the quantitativity; however, it is usually challenging. In this study, we utilized a cyclone flow device and established a liquid concentration method. An air cyclone flow induced a liquid cyclone flow in the concentration devices and enhanced the air/liquid interface area, which allowed an effective concentration of liquid from mL to mL at room temperature. The heating temperature and actual temperature inside the liquid were investigated to know the cooling effect of evaporation. The collection efficiency of larger than 98% was confirmed with a standard solution. Finally, the analytical procedure to realize a quantitative concentration was established, and the concentration and quantification of interleukins (IL-8, IL-17, and IL-23) from the supernatant of the B-cell culture medium was demonstrated. The B-cell was stimulated with CD40L, and the supernatant was concentrated 27 times at maximum.

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来源期刊
Analytical Sciences
Analytical Sciences 化学-分析化学
CiteScore
2.90
自引率
18.80%
发文量
232
审稿时长
1 months
期刊介绍: Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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