{"title":"校正物理交联聚乙烯醇细胞培养板涂层，促进细胞间相互作用、球形体形成和干性的保存","authors":"","doi":"10.1002/jbm.b.35506","DOIUrl":null,"url":null,"abstract":"<p>K. Molyneaux, M. D. Wnek, S. E. L. Craig, et al., “Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell–Cell Interactions, Spheroid Formation, and Stemness,” Journal of Biomedical Materials Research. Part B, Applied Biomaterials 109, no. 11 (2021):1744–1753.</p><p>FIGURE 4 Immunostaining of spheroids from long-term cultures indicate presence of various cell lineages and tumor biomarkers. Day 18 LN229 spheres derived from culture in 60 mm dishes were sectioned and processed for immunohistochemistry for neuronal, stem cell, glial, vascular, mesenchymal, and tumor biomarkers. LN229 spheres express markers of neuronal stem cells (FoxG1 and Zeb 1) and vascular (CD31) and mesenchymal (vimentin) populations but not glial cells (note absence of GFAP staining). There is also some expression of a neural marker (βIII tubulin). In addition, staining with a tumor biomarker to PTPμ, SBK4, was also present. A phase contrast image of an 18-day aggregate is also shown.</p><p>We apologize for this error.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35506","citationCount":"0","resultStr":"{\"title\":\"Correction to Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell–Cell Interactions, Spheroid Formation, and Stemness\",\"authors\":\"\",\"doi\":\"10.1002/jbm.b.35506\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>K. Molyneaux, M. D. Wnek, S. E. L. Craig, et al., “Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell–Cell Interactions, Spheroid Formation, and Stemness,” Journal of Biomedical Materials Research. Part B, Applied Biomaterials 109, no. 11 (2021):1744–1753.</p><p>FIGURE 4 Immunostaining of spheroids from long-term cultures indicate presence of various cell lineages and tumor biomarkers. Day 18 LN229 spheres derived from culture in 60 mm dishes were sectioned and processed for immunohistochemistry for neuronal, stem cell, glial, vascular, mesenchymal, and tumor biomarkers. LN229 spheres express markers of neuronal stem cells (FoxG1 and Zeb 1) and vascular (CD31) and mesenchymal (vimentin) populations but not glial cells (note absence of GFAP staining). There is also some expression of a neural marker (βIII tubulin). In addition, staining with a tumor biomarker to PTPμ, SBK4, was also present. A phase contrast image of an 18-day aggregate is also shown.</p><p>We apologize for this error.</p>\",\"PeriodicalId\":15269,\"journal\":{\"name\":\"Journal of biomedical materials research. Part B, Applied biomaterials\",\"volume\":\"112 12\",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35506\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biomedical materials research. Part B, Applied biomaterials\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.35506\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical materials research. Part B, Applied biomaterials","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.35506","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
摘要
K.Molyneaux, M. D. Wnek, S. E. L. Craig, et al., "Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell-Cell Interactions, Spheroid Formation, and Stemness," Journal of Biomedical Materials Research.图 4 长期培养球体的免疫染色表明存在各种细胞系和肿瘤生物标志物。对在 60 毫米培养皿中培养到第 18 天的 LN229 球体进行切片和免疫组化处理，以检测神经元、干细胞、胶质细胞、血管、间充质和肿瘤生物标志物。LN229 球体表达神经元干细胞（FoxG1 和 Zeb 1）、血管（CD31）和间充质（波形蛋白）标志物，但不表达神经胶质细胞（注意没有 GFAP 染色）。神经标记物（βⅢ微管蛋白）也有一定程度的表达。此外，PTPμ的肿瘤生物标记物SBK4也出现了染色。图中还显示了 18 天聚合体的相衬图像。
Correction to Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell–Cell Interactions, Spheroid Formation, and Stemness
K. Molyneaux, M. D. Wnek, S. E. L. Craig, et al., “Physically-Cross-Linked Poly(Vinyl Alcohol) Cell Culture Plate Coatings Facilitate Preservation of Cell–Cell Interactions, Spheroid Formation, and Stemness,” Journal of Biomedical Materials Research. Part B, Applied Biomaterials 109, no. 11 (2021):1744–1753.
FIGURE 4 Immunostaining of spheroids from long-term cultures indicate presence of various cell lineages and tumor biomarkers. Day 18 LN229 spheres derived from culture in 60 mm dishes were sectioned and processed for immunohistochemistry for neuronal, stem cell, glial, vascular, mesenchymal, and tumor biomarkers. LN229 spheres express markers of neuronal stem cells (FoxG1 and Zeb 1) and vascular (CD31) and mesenchymal (vimentin) populations but not glial cells (note absence of GFAP staining). There is also some expression of a neural marker (βIII tubulin). In addition, staining with a tumor biomarker to PTPμ, SBK4, was also present. A phase contrast image of an 18-day aggregate is also shown.
期刊介绍：
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats:
• original research reports
• short research and development reports
• scientific reviews
• current concepts articles
• special reports
• editorials
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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