Xingzhao Sun, Amine M’Barek Bouanane, Leonardo H. Teixeira, Judith Sitters, Harry Olde Venterink
{"title":"食草动物粪便中的微生物活动影响毛地黄的结瘤和生长","authors":"Xingzhao Sun, Amine M’Barek Bouanane, Leonardo H. Teixeira, Judith Sitters, Harry Olde Venterink","doi":"10.1007/s11104-024-07089-5","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background and aims</h3><p>Herbivore dung varies among species in terms of nutrients and microbial composition, but the effect of the microbial activity in the dung on plant growth and symbiotic association is largely unknown. This study aimed to investigate the influence of cow dung quantity and dung microbial activity on plant-microbe interactions (mycorrhiza, nodulation) and the growth of <i>Trifolium pratense</i> seedlings.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>A mesocosm experiment was conducted with gradients of unsterilized or sterilized cow dung applied to pots with <i>T. pratense</i> seedlings. Biomass, relative growth rate (RGR), root phosphomonoesterase (PME) activity, nodulation, and root mycorrhizal colonization of the seedling were measured after 7 weeks.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>Growth of <i>T. pratense</i> increased with increasing dung supply, while sterilization marginally decreased growth by 15–20% (<i>P</i> = 0.068). Root nodulation increased with increasing dung supply but was significantly lower with sterilized dung. Both root PME activity and mycorrhizal colonization decreased with increasing dung supply but were unaffected by sterilization.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>The decreased growth and nodulation due to dung sterilization aligned with our predictions but could not be attributed to reduced dung decomposition and nutrient availability. Instead, the reduced microbial activity from sterilization likely negatively impacted bacterial N<sub>2</sub>-fixing activity and, consequently, plant growth. This suggests that herbivore dung’s effect on plant growth and interactions is more complex than previously anticipated, influenced not only by nutrient supply and stoichiometry but also by microbial composition and activity.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"99 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microbial activity in herbivore dung affects nodulation and growth of Trifolium pratense\",\"authors\":\"Xingzhao Sun, Amine M’Barek Bouanane, Leonardo H. Teixeira, Judith Sitters, Harry Olde Venterink\",\"doi\":\"10.1007/s11104-024-07089-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Background and aims</h3><p>Herbivore dung varies among species in terms of nutrients and microbial composition, but the effect of the microbial activity in the dung on plant growth and symbiotic association is largely unknown. This study aimed to investigate the influence of cow dung quantity and dung microbial activity on plant-microbe interactions (mycorrhiza, nodulation) and the growth of <i>Trifolium pratense</i> seedlings.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>A mesocosm experiment was conducted with gradients of unsterilized or sterilized cow dung applied to pots with <i>T. pratense</i> seedlings. Biomass, relative growth rate (RGR), root phosphomonoesterase (PME) activity, nodulation, and root mycorrhizal colonization of the seedling were measured after 7 weeks.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>Growth of <i>T. pratense</i> increased with increasing dung supply, while sterilization marginally decreased growth by 15–20% (<i>P</i> = 0.068). Root nodulation increased with increasing dung supply but was significantly lower with sterilized dung. Both root PME activity and mycorrhizal colonization decreased with increasing dung supply but were unaffected by sterilization.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusions</h3><p>The decreased growth and nodulation due to dung sterilization aligned with our predictions but could not be attributed to reduced dung decomposition and nutrient availability. Instead, the reduced microbial activity from sterilization likely negatively impacted bacterial N<sub>2</sub>-fixing activity and, consequently, plant growth. This suggests that herbivore dung’s effect on plant growth and interactions is more complex than previously anticipated, influenced not only by nutrient supply and stoichiometry but also by microbial composition and activity.</p>\",\"PeriodicalId\":20223,\"journal\":{\"name\":\"Plant and Soil\",\"volume\":\"99 1\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-11-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant and Soil\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11104-024-07089-5\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-024-07089-5","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Microbial activity in herbivore dung affects nodulation and growth of Trifolium pratense
Background and aims
Herbivore dung varies among species in terms of nutrients and microbial composition, but the effect of the microbial activity in the dung on plant growth and symbiotic association is largely unknown. This study aimed to investigate the influence of cow dung quantity and dung microbial activity on plant-microbe interactions (mycorrhiza, nodulation) and the growth of Trifolium pratense seedlings.
Methods
A mesocosm experiment was conducted with gradients of unsterilized or sterilized cow dung applied to pots with T. pratense seedlings. Biomass, relative growth rate (RGR), root phosphomonoesterase (PME) activity, nodulation, and root mycorrhizal colonization of the seedling were measured after 7 weeks.
Results
Growth of T. pratense increased with increasing dung supply, while sterilization marginally decreased growth by 15–20% (P = 0.068). Root nodulation increased with increasing dung supply but was significantly lower with sterilized dung. Both root PME activity and mycorrhizal colonization decreased with increasing dung supply but were unaffected by sterilization.
Conclusions
The decreased growth and nodulation due to dung sterilization aligned with our predictions but could not be attributed to reduced dung decomposition and nutrient availability. Instead, the reduced microbial activity from sterilization likely negatively impacted bacterial N2-fixing activity and, consequently, plant growth. This suggests that herbivore dung’s effect on plant growth and interactions is more complex than previously anticipated, influenced not only by nutrient supply and stoichiometry but also by microbial composition and activity.
期刊介绍：
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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