基于贻贝启发的聚（去甲肾上腺素）和功能肽的防污电化学生物传感器用于检测血清中的细胞外信号调节激酶 2

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-11-19 DOI:10.1016/j.snb.2024.136964

Hao Zhou, Xiqin Yang, Mingjun Shi, Xijuan Yu, Xiliang Luo

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引用次数: 0

摘要

非特异性生物分子在传感表面的吸附对准确灵敏地检测复杂生物样本中的目标物构成了挑战，这极大地限制了生物传感器的实际应用。因此，开发具有防污功能的传感设备至关重要。在此，我们利用两种防污材料开发了一种低污损电化学生物传感平台。一种材料是专门设计的功能肽，另一种材料是受贻贝粘附特性启发而设计的聚去甲肾上腺素（PNE）。与聚多巴胺相比，PNE 能更有效地减少血清样本中的非特异性吸附。利用 PNE 和多肽开发的生物传感器具有优异的抗污能力，在检测目标细胞外信号调节激酶 2（ERK2）时，衬垫检测范围宽达 10.0 pg-mL-1 - 10.0 µg-mL-1，可在较宽的浓度范围内进行精确测量，检测限为 3.97 pg-mL-1。这种防污生物传感器无需预处理就能检测血清中的目标 ERK2，准确度与 ELISA 试剂盒相当。这种利用多肽和 PNE 构建低污损生物传感器的方法可以很容易地扩展到开发其他传感系统，用于分析人体液中的各种目标物。

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Antifouling electrochemical biosensors based on mussel-inspired poly(norepinephrine) and functional peptides for the detection of extracellular signal-regulated kinase 2 in serum

The adsorption of nonspecific biomolecules on sensing surfaces presents a challenge for accurate and sensitive detection of targets in complex biological samples, which significantly restricts the practical application of biosensors. It is therefore crucial to develop sensing devices with antifouling capabilities. Herein, a low fouling electrochemical biosensing platform has been developed that leverage two kinds of antifouling materials. One material employed is a specially designed functional peptide, while the other is inspired by the adhesive properties of mussels, known as poly(noradrenaline) (PNE). Compared with polydopamine, the PNE is more effective in reducing the nonspecific adsorption in serum samples. The biosensor, which was developed using PNE and peptide, demonstrated exceptional resistance to fouling and a broad liner detection range of 10.0 pg·mL⁻¹ - 10.0 µg·mL⁻¹ for the detection of the target extracellular signal-regulated kinase 2 (ERK2), allowing for accurate measurements over a broad range of concentrations, with a limit of detection of 3.97 pg·mL⁻¹. The antifouling biosensor was able to detect target ERK2 in serum without pretreatment, and with satisfying accuracy comparable to the ELISA kit. This approach for the construction of low fouling biosensors using peptides and PNE can be easily extended to develop other sensing systems for various targets analysis in human body fluids.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.