利用 DNAzyme 纳米机器人原位成像抗生素暴露下的细胞炎症反应

IF 10.8 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Aijiao Yuan, Huifang Hao, Rui Sha, Hang Xiao, Fan Yang, Bo Pang, Jinhua Li*, Minghua Jin, Wenjing Xie, Lixia Zhao, Yawei Wang, Yu Zhang, Juan Li and Hanyong Peng*, 
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引用次数: 0

摘要

抗生素诱发的炎症涉及髓过氧化物酶(MPO)的释放,这种酶在组织中的表达与炎症途径有关。然而,检测细胞中 MPO 的现有方法非常有限。本研究开发了一种 DNA 酶纳米机器人,其支架是用功能性 DNA 酶链及其荧光团标记的底物链装饰的金纳米粒子(AuNPs)。DNA 酶由于自组装的发夹结构而保持非活性,茎域中插入了硫代磷酸酯(PT)修饰。当 MPO 存在时,它会触发卤化过程,生成次氯酸(HClO)。HClO 能特异性地催化 PT 位点的裂解,释放出游离的 DNA 酶链以裂解其底物,并产生不断增强的荧光信号。MPO 及其主要产物 HClO 的检测限分别为 0.038 μg/mL 和 0.013 μM。DNA 酶纳米机器人可以很容易地导入细胞,并自主发挥作用,以区分抗生素引起的 MPO/HClO 水平升高。这种方法被用于对暴露于环境中四种常见抗生素(12-肉豆蔻酸 13-乙酸磷脂、红霉素、青霉素和四环素)以及抗生素生产废水中的 RAW264.7 细胞进行成像。这种纳米机器人提供了监测炎症的新策略,以评估抗生素暴露对健康的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In Situ Imaging of Cellular Inflammatory Response to Antibiotic Exposure with a DNAzyme Nanorobot

In Situ Imaging of Cellular Inflammatory Response to Antibiotic Exposure with a DNAzyme Nanorobot

Antibiotic-induced inflammation involves the release of myeloperoxidase (MPO), an enzyme whose expression in tissues is associated with the inflammatory pathway. However, existing methods for detecting MPO in cells are limited. In this study, a DNAzyme nanorobot was developed using a scaffold of gold nanoparticles (AuNPs) decorated with functional DNAzyme strands and their fluorophore-labeled substrate strands. The DNAzyme remains inactive due to a self-assembled hairpin structure, with a phosphorothioate (PT) modification inserted into the stem domain. When MPO is present, it triggers a halogenation process that generates hypochlorous acid (HClO). HClO specifically catalyzes the cleavage of the PT-site, releasing free DNAzyme strands to cleave their substrates and generating an increasing fluorescent signal. The detection limit for MPO and its primary product, HClO, were determined to be 0.038 μg/mL and 0.013 μM, respectively. The DNAzyme nanorobot can be readily introduced into cells and function autonomously to differentiate increased MPO/HClO levels caused by antibiotics. This approach was applied to image RAW264.7 cells exposed to four prevalent antibiotics found in the environment (phorbol 12-myristate 13-acetate, erythromycin, penicillin, and tetracycline) as well as antibiotic production wastewater. This nanorobot offers novel strategies for monitoring inflammation to evaluate the health impacts of antibiotic exposure.

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来源期刊
环境科学与技术
环境科学与技术 环境科学-工程:环境
CiteScore
17.50
自引率
9.60%
发文量
12359
审稿时长
2.8 months
期刊介绍: Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences. Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.
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