Hui Yang, Yihan Zhang, Yushan Zhao, Yinping Shu, Yushu Xu, Yi Liu, Junbo Du, Wenming Wang
{"title":"通过宿主诱导的两个分泌基因沉默，减少 Plasmodiophora brassicae 对 Brassica rapa 的感染。","authors":"Hui Yang, Yihan Zhang, Yushan Zhao, Yinping Shu, Yushu Xu, Yi Liu, Junbo Du, Wenming Wang","doi":"10.1094/PHYTO-09-23-0334-R","DOIUrl":null,"url":null,"abstract":"<p><p>Clubroot disease caused by the biotrophic pathogen <i>Plasmodiophora brassicae</i>, is one of the most serious threats to cruciferous crops production worldwide. <i>P. brassicae</i> is known for rapid adaptive evolution to overcome resistance in varieties. It is urgent to establish alternative management to control <i>P. brassicae</i>. In this study, we identified two <i>P. brassicae</i> secretory proteins that were up-regulated during infection and effected plant defense. We established a method for transient expression in the roots of seedlings and demonstrated that <i>P. brassicae</i> could take up substances from the environment of root cells. Using a RNA interference (RNAi)-based host-induced gene silencing (HIGS) by expression of hairpin RNAi constructs with sequence homology to <i>P. brassicae</i> effector <i>Pb48</i> or <i>Pb52</i> in susceptible <i>Brassica rapa</i> plants enhanced host disease resistance. After silencing these two effectors, the transcription levels of cytokinin biosynthesis gene <i>IPT1</i> and the regulation gene of auxin homeostasis <i>GH3.5</i> were down-regulated. These results suggested that RNAi-based HIGS of effectors has a great practical application of improving crop resistance against <i>P. brassicae</i> and can contribute to environmentally sustainable agriculture.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Reduction of <i>Plasmodiophora brassicae</i> infection on <i>Brassica rapa</i> through host-induced gene silencing of two secreted genes.\",\"authors\":\"Hui Yang, Yihan Zhang, Yushan Zhao, Yinping Shu, Yushu Xu, Yi Liu, Junbo Du, Wenming Wang\",\"doi\":\"10.1094/PHYTO-09-23-0334-R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Clubroot disease caused by the biotrophic pathogen <i>Plasmodiophora brassicae</i>, is one of the most serious threats to cruciferous crops production worldwide. <i>P. brassicae</i> is known for rapid adaptive evolution to overcome resistance in varieties. It is urgent to establish alternative management to control <i>P. brassicae</i>. In this study, we identified two <i>P. brassicae</i> secretory proteins that were up-regulated during infection and effected plant defense. We established a method for transient expression in the roots of seedlings and demonstrated that <i>P. brassicae</i> could take up substances from the environment of root cells. Using a RNA interference (RNAi)-based host-induced gene silencing (HIGS) by expression of hairpin RNAi constructs with sequence homology to <i>P. brassicae</i> effector <i>Pb48</i> or <i>Pb52</i> in susceptible <i>Brassica rapa</i> plants enhanced host disease resistance. After silencing these two effectors, the transcription levels of cytokinin biosynthesis gene <i>IPT1</i> and the regulation gene of auxin homeostasis <i>GH3.5</i> were down-regulated. These results suggested that RNAi-based HIGS of effectors has a great practical application of improving crop resistance against <i>P. brassicae</i> and can contribute to environmentally sustainable agriculture.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-11-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-09-23-0334-R\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-09-23-0334-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Reduction of Plasmodiophora brassicae infection on Brassica rapa through host-induced gene silencing of two secreted genes.
Clubroot disease caused by the biotrophic pathogen Plasmodiophora brassicae, is one of the most serious threats to cruciferous crops production worldwide. P. brassicae is known for rapid adaptive evolution to overcome resistance in varieties. It is urgent to establish alternative management to control P. brassicae. In this study, we identified two P. brassicae secretory proteins that were up-regulated during infection and effected plant defense. We established a method for transient expression in the roots of seedlings and demonstrated that P. brassicae could take up substances from the environment of root cells. Using a RNA interference (RNAi)-based host-induced gene silencing (HIGS) by expression of hairpin RNAi constructs with sequence homology to P. brassicae effector Pb48 or Pb52 in susceptible Brassica rapa plants enhanced host disease resistance. After silencing these two effectors, the transcription levels of cytokinin biosynthesis gene IPT1 and the regulation gene of auxin homeostasis GH3.5 were down-regulated. These results suggested that RNAi-based HIGS of effectors has a great practical application of improving crop resistance against P. brassicae and can contribute to environmentally sustainable agriculture.
期刊介绍：
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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