从海洋环境中分离的 Roseicyclus sediminis sp.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2024-11-01 DOI:10.1099/ijsem.0.006583

Ziyang Zhou, Xunian Yang, Yuyan Yue, Xinjiang Liu, Zhenxing Xu, Zongjun Du

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Based on 16S rRNA gene sequence analysis, the closest relatives of strains SDUM158016T and SDUM158017T are 'Roseibacterium beibuensis' MCCC 1F00103T, followed by Roseibacterium elongatum JCM 11220T, with the highest similarities 97.1 and 97.3 %, respectively. The average nucleotide identity values between strain SDUM158016T and species of the genera Roseibacterium and Roseicyclus ranged from 76.0 to 78.9 %, while they ranged from 75.9 to 78.6% for strain SDUM158017T. These values are below the species delineation threshold of 98.6%, suggesting that both strains represent novel species. Phylogenetic analysis of the 16S rRNA genes and genome sequences further indicates that Roseibacterium species within the genus Roseicyclus and these isolates represent two potentially novel species within the genus Roseicyclus. 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引用次数: 0

摘要

从中国威海沿海沉积物和盐沼中分离出两种革兰氏染色阴性、兼性厌氧、杆状细菌，分别命名为 SDUM158017T 和 SDUM158016T。这两种细菌都能在 20 至 43°C 的温度和 6.0 至 9.0 的 pH 值范围内生长。不过，菌株 SDUM158016T 在 1.0-7.0%（w/v）NaCl 的条件下生长，而菌株 SDUM158017T 则能在 1.0-10.0%（w/v）NaCl 的条件下生长。菌株 SDUM158016T 和 SDUM158017T 含有 C18 ：1 ω7ϲ 和 C16 ：0 并含有 Q10 作为唯一的醌。根据 16S rRNA 基因序列分析，SDUM158016T 和 SDUM158017T 的近亲是'Roseibacterium beibuensis' MCCC 1F00103T，其次是 Roseibacterium elongatum JCM 11220T，相似度最高，分别为 97.1 % 和 97.3 %。菌株 SDUM158016T 与 Roseibacterium 属和 Roseicyclus 属物种的平均核苷酸同一性值介于 76.0% 与 78.9% 之间，而菌株 SDUM158017T 的同一性值介于 75.9% 与 78.6% 之间。这些数值低于 98.6% 的物种划分阈值，表明这两株菌株代表了新物种。16S rRNA 基因和基因组序列的系统进化分析进一步表明，Roseibacterium 是 Roseicyclus 属中的一个种，而这些分离株代表了 Roseicyclus 属中的两个潜在新种。综合来看，菌株 SDUM158017T 和 SDUM158016T 代表了 Roseicyclus 属的两个新种，并将其命名为 Roseicyclus sediminis 和 Roseicyclus salinarum，模式菌株分别为 SDUM158016T（=KCTC 92632T =MCCC 1H01367T）和 SDUM158017T（=KCTC 92633T =MCCC 1H01363T）。基于系统发育和基因组分析，我们还建议将 Roseibacterium elongatum 重新分类为 Roseicyclus elongatus comb.

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Roseicyclus sediminis sp. nov. and Roseicyclus salinarum sp. nov., isolated from marine environment, and reclassification of two species of the genus Roseibacterium as species of the genus Roseicyclus.

Two Gram-stain-negative, facultatively anaerobic, rod-shaped bacteria were isolated from coastal sediments and salt marshes in Weihai, China, and designated as SDUM158017^T and SDUM158016^T, respectively. Both grew at temperatures ranging from 20 to 43°C and within a pH range of 6.0 to 9.0. However, strain SDUM158016^T grew in the presence of 1.0-7.0% (w/v) NaCl, whereas strain SDUM158017^T was able to grow in the presence of 1.0-10.0% (w/v) NaCl. Strains SDUM158016^T and SDUM158017^T contain major fatty acids of C_18 : 1 ω7ϲ and C_16 : 0 and contain Q10 as the sole quinone. Based on 16S rRNA gene sequence analysis, the closest relatives of strains SDUM158016^T and SDUM158017^T are 'Roseibacterium beibuensis' MCCC 1F00103^T, followed by Roseibacterium elongatum JCM 11220^T, with the highest similarities 97.1 and 97.3 %, respectively. The average nucleotide identity values between strain SDUM158016^T and species of the genera Roseibacterium and Roseicyclus ranged from 76.0 to 78.9 %, while they ranged from 75.9 to 78.6% for strain SDUM158017^T. These values are below the species delineation threshold of 98.6%, suggesting that both strains represent novel species. Phylogenetic analysis of the 16S rRNA genes and genome sequences further indicates that Roseibacterium species within the genus Roseicyclus and these isolates represent two potentially novel species within the genus Roseicyclus. Taken together, strains SDUM158017^T and SDUM158016^T represent two novel species of the genus Roseicyclus, for which the names Roseicyclus sediminis and Roseicyclus salinarum are proposed with the type strains SDUM158016^T (=KCTC 92632^T =MCCC 1H01367^T) and SDUM158017^T (=KCTC 92633^T =MCCC 1H01363^T), respectively. Based on phylogenetic and genomic analyses, we also propose the reclassification of Roseibacterium elongatum as Roseicyclus elongatus comb. nov. and Roseibacterium persicicum as Roseicyclus persicicus comb. nov.

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.