{"title":"Bulbophyllum kwangtumgense Schltr.","authors":"Liuyue Xiao, Jiaojiao Hou, Ping Chen, Xiao Tan, Xiangxiang Qin, Jing Nie, Hua Zhu, Shian Zhong","doi":"10.1016/j.ijbiomac.2024.137382","DOIUrl":null,"url":null,"abstract":"<p><p>A microwave-assisted method was used to extract the polysaccharide from Bulbophyllumkwangtumgense Schltr, and the optimum extraction conditions were determined by orthogonal experiments. The purified polysaccharide component BKP-1 had an obvious polysaccharide characteristic structure on the infrared spectrum and was mainly composed of xylose and glucuronic acid at a ratio of 0.568:0.432. Its molecular weight distribution (polydispersity index was 0.58) was uniform with 1.92× 10<sup>6</sup> Da. The main structural skeleton of BKP-1 was →4)-β-D-Xylp-(1 → [4)-β-D-Xylp-(1]<sub>4</sub> → 4)-α-D-GlcAp-(1 → [4)-α-D-GlcAp-(1]<sub>2</sub>→. BKP-1 could clear certain free radicals, among which the hydroxyl free radical scavenging ability was the best, with a clearance rate of 61.46 %. Furthermore, BKP-1 significantly modulated the secretion of both pro-inflammatory and anti-inflammatory cytokines at a concentration of 125 μg/mL. Notably, the mechanism BKP-1 exhibiting inhibitory effects were related to regulating of the NF-κB signaling pathway.</p>","PeriodicalId":333,"journal":{"name":"International Journal of Biological Macromolecules","volume":" ","pages":"137382"},"PeriodicalIF":7.7000,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation, characterization, and activity of the polysaccharides in Bulbophyllum kwangtumgense Schltr.\",\"authors\":\"Liuyue Xiao, Jiaojiao Hou, Ping Chen, Xiao Tan, Xiangxiang Qin, Jing Nie, Hua Zhu, Shian Zhong\",\"doi\":\"10.1016/j.ijbiomac.2024.137382\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A microwave-assisted method was used to extract the polysaccharide from Bulbophyllumkwangtumgense Schltr, and the optimum extraction conditions were determined by orthogonal experiments. The purified polysaccharide component BKP-1 had an obvious polysaccharide characteristic structure on the infrared spectrum and was mainly composed of xylose and glucuronic acid at a ratio of 0.568:0.432. Its molecular weight distribution (polydispersity index was 0.58) was uniform with 1.92× 10<sup>6</sup> Da. The main structural skeleton of BKP-1 was →4)-β-D-Xylp-(1 → [4)-β-D-Xylp-(1]<sub>4</sub> → 4)-α-D-GlcAp-(1 → [4)-α-D-GlcAp-(1]<sub>2</sub>→. BKP-1 could clear certain free radicals, among which the hydroxyl free radical scavenging ability was the best, with a clearance rate of 61.46 %. Furthermore, BKP-1 significantly modulated the secretion of both pro-inflammatory and anti-inflammatory cytokines at a concentration of 125 μg/mL. Notably, the mechanism BKP-1 exhibiting inhibitory effects were related to regulating of the NF-κB signaling pathway.</p>\",\"PeriodicalId\":333,\"journal\":{\"name\":\"International Journal of Biological Macromolecules\",\"volume\":\" \",\"pages\":\"137382\"},\"PeriodicalIF\":7.7000,\"publicationDate\":\"2024-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biological Macromolecules\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ijbiomac.2024.137382\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological Macromolecules","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.ijbiomac.2024.137382","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Isolation, characterization, and activity of the polysaccharides in Bulbophyllum kwangtumgense Schltr.
A microwave-assisted method was used to extract the polysaccharide from Bulbophyllumkwangtumgense Schltr, and the optimum extraction conditions were determined by orthogonal experiments. The purified polysaccharide component BKP-1 had an obvious polysaccharide characteristic structure on the infrared spectrum and was mainly composed of xylose and glucuronic acid at a ratio of 0.568:0.432. Its molecular weight distribution (polydispersity index was 0.58) was uniform with 1.92× 106 Da. The main structural skeleton of BKP-1 was →4)-β-D-Xylp-(1 → [4)-β-D-Xylp-(1]4 → 4)-α-D-GlcAp-(1 → [4)-α-D-GlcAp-(1]2→. BKP-1 could clear certain free radicals, among which the hydroxyl free radical scavenging ability was the best, with a clearance rate of 61.46 %. Furthermore, BKP-1 significantly modulated the secretion of both pro-inflammatory and anti-inflammatory cytokines at a concentration of 125 μg/mL. Notably, the mechanism BKP-1 exhibiting inhibitory effects were related to regulating of the NF-κB signaling pathway.
期刊介绍：
The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
