分子氢对根的向心力有积极影响,其中涉及辅助素信号转导和淀粉积累。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Yingying Zhang, Ziyu Liu, Huize Huang, Longna Li, Sheng Xu, Wenbiao Shen
{"title":"分子氢对根的向心力有积极影响,其中涉及辅助素信号转导和淀粉积累。","authors":"Yingying Zhang, Ziyu Liu, Huize Huang, Longna Li, Sheng Xu, Wenbiao Shen","doi":"10.1111/tpj.17151","DOIUrl":null,"url":null,"abstract":"<p><p>Although geoscience of natural hydrogen (H<sub>2</sub>), hydrogen-producing soil bacteria, and especially plant-based H<sub>2</sub>, has been observed, it is not clear whether or how above H<sub>2</sub> resources influence root gravitropic responses. Here, pharmacological, genetic, molecular, and cell biological tools were applied to investigate how plant-based H<sub>2</sub> coordinates gravity responses in Arabidopsis roots. Since roots show higher H<sub>2</sub> production than shoots, exogenous H<sub>2</sub> supply was used to mimic this function. After H<sub>2</sub> supplementation, the asymmetric expression of the auxin-response reporter DR5 driven by auxin influx and efflux carriers, and thereafter positive root gravitropism were observed. These positive responses in root gravitropism were sensitive to auxin polar transport inhibitors, and importantly, the defective phenotypes observed in aux1-7, pin1, and pin2 mutants were not significantly altered by exogenous H<sub>2</sub>. The observed starch accumulation was matched with the reprogramming gene expression linked to starch synthesis and degradation. Transgenic plants expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only displayed higher endogenous H<sub>2</sub> concentrations, the inducible AUX1 gene expression and starch accumulation, but also showed pronounced root gravitropism. Collectively, above evidence preliminarily provides a framework for understanding the molecular basis of the possible functions of both plant/soil-based and nature H<sub>2</sub> in root architecture.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular hydrogen positively influences root gravitropism involving auxin signaling and starch accumulation.\",\"authors\":\"Yingying Zhang, Ziyu Liu, Huize Huang, Longna Li, Sheng Xu, Wenbiao Shen\",\"doi\":\"10.1111/tpj.17151\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Although geoscience of natural hydrogen (H<sub>2</sub>), hydrogen-producing soil bacteria, and especially plant-based H<sub>2</sub>, has been observed, it is not clear whether or how above H<sub>2</sub> resources influence root gravitropic responses. Here, pharmacological, genetic, molecular, and cell biological tools were applied to investigate how plant-based H<sub>2</sub> coordinates gravity responses in Arabidopsis roots. Since roots show higher H<sub>2</sub> production than shoots, exogenous H<sub>2</sub> supply was used to mimic this function. After H<sub>2</sub> supplementation, the asymmetric expression of the auxin-response reporter DR5 driven by auxin influx and efflux carriers, and thereafter positive root gravitropism were observed. These positive responses in root gravitropism were sensitive to auxin polar transport inhibitors, and importantly, the defective phenotypes observed in aux1-7, pin1, and pin2 mutants were not significantly altered by exogenous H<sub>2</sub>. The observed starch accumulation was matched with the reprogramming gene expression linked to starch synthesis and degradation. Transgenic plants expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only displayed higher endogenous H<sub>2</sub> concentrations, the inducible AUX1 gene expression and starch accumulation, but also showed pronounced root gravitropism. Collectively, above evidence preliminarily provides a framework for understanding the molecular basis of the possible functions of both plant/soil-based and nature H<sub>2</sub> in root architecture.</p>\",\"PeriodicalId\":233,\"journal\":{\"name\":\"The Plant Journal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2024-11-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Journal\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://doi.org/10.1111/tpj.17151\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/tpj.17151","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

虽然人们已经观察到了天然氢气(H2)、产氢土壤细菌,特别是植物源氢气的地球科学特性,但尚不清楚上述氢气资源是否或如何影响根系的重力反应。在此,研究人员应用药理学、遗传学、分子和细胞生物学工具研究了植物基 H2 如何协调拟南芥根系的重力反应。由于根的 H2 产量比芽高,因此采用外源 H2 供应来模拟这一功能。在补充 H2 后,观察到了由辅酶流入和流出载体驱动的辅酶反应报告物 DR5 的不对称表达,以及随后根的正向引力。根引力的这些正反应对辅素极性运输抑制剂很敏感,而且重要的是,在 aux1-7、pin1 和 pin2 突变体中观察到的缺陷表型并未因外源 H2 而发生显著改变。观察到的淀粉积累与与淀粉合成和降解相关的基因表达重编程相匹配。表达莱茵衣藻氢化酶 1(CrHYD1)的转基因植株不仅表现出较高的内源 H2 浓度、诱导性 AUX1 基因表达和淀粉积累,还表现出明显的根引力。总之,上述证据初步为了解植物/土壤和自然界的 H2 在根系结构中可能发挥的功能的分子基础提供了一个框架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular hydrogen positively influences root gravitropism involving auxin signaling and starch accumulation.

Although geoscience of natural hydrogen (H2), hydrogen-producing soil bacteria, and especially plant-based H2, has been observed, it is not clear whether or how above H2 resources influence root gravitropic responses. Here, pharmacological, genetic, molecular, and cell biological tools were applied to investigate how plant-based H2 coordinates gravity responses in Arabidopsis roots. Since roots show higher H2 production than shoots, exogenous H2 supply was used to mimic this function. After H2 supplementation, the asymmetric expression of the auxin-response reporter DR5 driven by auxin influx and efflux carriers, and thereafter positive root gravitropism were observed. These positive responses in root gravitropism were sensitive to auxin polar transport inhibitors, and importantly, the defective phenotypes observed in aux1-7, pin1, and pin2 mutants were not significantly altered by exogenous H2. The observed starch accumulation was matched with the reprogramming gene expression linked to starch synthesis and degradation. Transgenic plants expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only displayed higher endogenous H2 concentrations, the inducible AUX1 gene expression and starch accumulation, but also showed pronounced root gravitropism. Collectively, above evidence preliminarily provides a framework for understanding the molecular basis of the possible functions of both plant/soil-based and nature H2 in root architecture.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信