Dr. Cesar Andres Diaz Arias, Prof. Dr. Marcelo Chuei Matsudo, Prof. Dr. Livia Seno Ferreira-Camargo, Dr. João Vitor Dutra Molino, Prof. Dr. Stephen Patrick Mayfield, Prof. Dr. João Carlos Monteiro de Carvalho
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Effect of Macronutrients on Recombinant mCherry Production by Microalga
This study sought to evaluate the influence of five macronutrients (acetate, calcium, sulfate, nitrogen, and phosphate) on the cell growth and heterologous mCherry protein production by Chlamydomonas reinhardtii. In the first step, three nitrogen (N) sources were tested (NH4NO3, NaNO3, and NH4Cl), and NH4NO3 was selected as N source for the following step, due to the best results for mCherry protein production. In the second step, a central composite design 25 was employed to evaluate the effect of the five macronutrients. Although all nutrients had effect on maximum biomass concentration, only acetate, nitrogen, and phosphate showed to influence mCherry protein production. By employing the experimental design with small-scale culture in multiplates and multivariable regression, it was possible to achieve 12 % increase for recombinant protein production.
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