机器人辅助下的棒状杆菌菌株库表型分析,实现下一代原料的价值化

Q1 Environmental Science
Tamara Weiß, Fabian Stefan Franz Hartmann, Gerd Michael Seibold
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引用次数: 0

摘要

鉴定能够有效利用不同碳源(糖、糖酸和糖醇)并耐受水解物中抑制性副产物的微生物,是利用木质纤维素生物质(LCB)进行高效生物生产所面临的一项挑战。本研究开发了一种机器人辅助筛选方法,以鉴定 32 个放线菌类型的菌株,这些菌株主要属于棒状杆菌属。利用机器人辅助复制电镀,评估了 12 种不同碳源的使用情况以及对 LCB 衍生的主要抑制剂的耐受程度。在对底物利用情况和抑制剂耐受性进行综合分析的基础上,选出了五株菌株,以超滤亚硫酸废液(SSL)为唯一底物,在微型培养系统中进行进一步鉴定。值得注意的是,在这些实验中,即使在高浓度的亚硫酸盐废液中,Corynebacterium sp.
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Robot-assisted phenotyping of a Corynebacterium strain library towards valorization of next-generation feedstocks

Robot-assisted phenotyping of a Corynebacterium strain library towards valorization of next-generation feedstocks
The identification of microorganisms capable of efficiently using the different carbon sources (sugars, sugar acids, and sugar alcohols) and tolerating inhibitory by-products in hydrolysates is a challenge for the development of efficient bioproduction from lignocellulosic biomass (LCB). In this study, a robot-assisted screening approach was developed to characterize a collection of 32 Actinomycetes type strains, mainly belonging to the genus Corynebacterium. Using robot-assisted replica plating, the use of 12 different carbon-sources as well as the level of tolerance against prominent inhibitors derived from LCB was assessed. Based on a combined analysis of substrate utilization profiles and inhibitor tolerance five strains were chosen for further characterization in a miniaturised cultivation system using ultrafiltrated spent sulfite liquor (SSL) as the sole substrate. Noteworthy, in these experiments Corynebacterium sp. ATCC 14747 showed fast growth and biomass accumulation even in presence of high SSL concentrations.
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来源期刊
Bioresource Technology Reports
Bioresource Technology Reports Environmental Science-Environmental Engineering
CiteScore
7.20
自引率
0.00%
发文量
390
审稿时长
28 days
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