在 ORAC 试验中使用曙红-Y 和曙红-B 作为荧光探针，测定依达拉奉、槲皮素和杨梅素涉及概率波动的抗氧化能力

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2024-11-08 DOI:10.1016/j.jphotochem.2024.116142

Miwa Takatsuka , Satoru Goto , Kota Moritake , Yosuke Shimada , Tomohiro Tsuchida

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引用次数: 0

摘要

氧自由基吸收能力（ORAC）测定法通过过氧自由基相对于荧光探针的竞争性消耗来测量抗氧化剂的抗氧化活性。本研究利用量子计算对 ORAC 试验中的荧光素（FLH）、曙红 Y 和曙红 B 进行了评估。在 ORAC-FLH 试验中，曲洛毒素（TRO）和抗坏血酸（ASC）显示出滞后的荧光衰减动力学，阻断了淬灭级联中的初始反应。曙红对过氧自由基的反应性更强，因此在 ORAC-曙红测定中无法对 TRO 进行评估。槲皮素和杨梅素的曲线漂移与孵育时间的平方和 3/2 排序成正比，表明荧光衰减具有概率性。依达拉奉（EDA）对初始反应有微弱的抑制作用，但随着时间的推移会加速淬灭。在 ORAC-曙红测定中，由于曙红反应活性高，类黄酮和 EDA 的表现难以区分。ORAC-FLH 对 BHT 的评估效果不明显，但半衰期的变化与剂量有关。这项研究表明，ORAC-曙红测定法具有广泛的应用前景和未来比较研究的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Antioxidant capacity of edaravone, quercetin, and myricetin involving probabilistic fluctuations using eosin-Y and eosin-B as fluorescent probes in the ORAC assay

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Antioxidant capacity of edaravone, quercetin, and myricetin involving probabilistic fluctuations using eosin-Y and eosin-B as fluorescent probes in the ORAC assay

The oxygen radical absorbance capacity (ORAC) assay measures the antioxidant activity of the antioxidants through competitive consumption of peroxyl radicals relative to a fluorescent probe. This study evaluated fluorescein (FLH), eosin Y, and eosin B in ORAC assays using quantum computations. In ORAC-FLH assays, trolox (TRO) and ascorbic acid (ASC) showed fluorescence decay kinetics with a lag time, blocking initial reactions in the quenching cascade. Eosins were more reactive towards peroxyl radicals, making TRO assessment infeasible in ORAC-eosin assays. Quercetin and myricetin exhibited sigmoidal curve drifts proportional to squared and 3/2-ordered incubation times, indicating probabilistic fluorescence decay. Edaravone (EDA) weakly inhibited initial reactions, with accelerated quenching over time. In ORAC-eosin assays, flavonoids and EDA showed indistinguishable behavior due to high eosin reactivity. ORAC-FLH did not significantly assess BHT, though a dose-dependent change in half-life was noted. This study suggests broad applications for ORAC-eosin assays and potential for future comparative research.

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.