负载有吡罗替尼的曲妥珠单抗功能化 SK-BR-3 细胞膜包裹介孔二氧化硅纳米粒子用于 HER-2 阳性乳腺癌的靶向治疗

IF 5.2 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Xing Liu, Wenwen Shen
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引用次数: 0

摘要

本研究制备了负载吡罗替尼的曲妥珠单抗功能化SK-BR-3细胞膜包裹介孔二氧化硅纳米颗粒(Tra-CM-MSN-PYR),用于HER2阳性乳腺癌的靶向治疗。透射电子显微镜(TEM)表征显示,MSN具有球形形态和介孔通道,Tra-CM-MSN的结构是成功包覆在MSN表面的细胞膜(CM)层。细胞摄取实验表明,FITC标记的Tra-CM-MSN能被SK-BR-3乳腺癌细胞摄取,这说明Tra-CM-MSN与CM-MSN和MSN相比具有良好的靶向能力。体内成像实验表明,FITC 标记的 Tra-CM-MSN 在肿瘤组织中有显著积累,进一步证明了 Tra-CM-MSN 具有卓越的靶向特性。细胞凋亡实验表明,Tra-CM-MSN-PYR 能明显抑制 SK-BR-3 乳腺癌细胞的增殖。体内动物实验结果也表明,Tra-CM-MSN-PYR 能明显抑制肿瘤生长。这些结果表明,Tra-CM-MSN-PYR 未来有可能用作 HER2 阳性乳腺癌的靶向疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Trastuzumab-functionalized SK-BR-3 cell membrane-wrapped mesoporous silica nanoparticles loaded with pyrotinib for the targeted therapy of HER-2-positive breast cancer

Trastuzumab-functionalized SK-BR-3 cell membrane-wrapped mesoporous silica nanoparticles loaded with pyrotinib for the targeted therapy of HER-2-positive breast cancer
In this study, the trastuzumab-functionalized SK-BR-3 cell membrane-wrapped mesoporous silica nanoparticles loaded with pyrotinib (Tra-CM-MSN-PYR) were prepared for targeted therapy of HER2-positive breast cancer. Transmission electron microscopy (TEM) characterization showed that MSN had a spherical morphology with mesoporous channels and that the structure of Tra-CM-MSN was a cell membrane (CM) layer successfully coated on the surface of MSN. A cellular uptake assay demonstrated that FITC-labeled Tra-CM-MSN were taken up by SK-BR-3 breast cancer cells, which illustrated that Tra-CM-MSN had good targeting ability compared with CM-MSN and MSN. In vivo imaging experiments demonstrated significant accumulation of FITC-labeled Tra-CM-MSN in tumor tissues, further proving that Tra-CM-MSN have superior targeting properties. Cell apoptosis experiments suggested that Tra-CM-MSN-PYR significantly inhibited the proliferation of SK-BR-3 breast cancer cells. The results of in vivo animal experiments also showed that Tra-CM-MSN-PYR significantly inhibited tumor growth. These results indicate that Tra-CM-MSN-PYR has potential application as a targeted therapy for HER2-positive breast cancer in the future.
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来源期刊
International Journal of Pharmaceutics: X
International Journal of Pharmaceutics: X Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
6.60
自引率
0.00%
发文量
32
审稿时长
24 days
期刊介绍: International Journal of Pharmaceutics: X offers authors with high-quality research who want to publish in a gold open access journal the opportunity to make their work immediately, permanently, and freely accessible. International Journal of Pharmaceutics: X authors will pay an article publishing charge (APC), have a choice of license options, and retain copyright. Please check the APC here. The journal is indexed in SCOPUS, PUBMED, PMC and DOAJ. The International Journal of Pharmaceutics is the second most cited journal in the "Pharmacy & Pharmacology" category out of 358 journals, being the true home for pharmaceutical scientists concerned with the physical, chemical and biological properties of devices and delivery systems for drugs, vaccines and biologicals, including their design, manufacture and evaluation. This includes evaluation of the properties of drugs, excipients such as surfactants and polymers and novel materials. The journal has special sections on pharmaceutical nanotechnology and personalized medicines, and publishes research papers, reviews, commentaries and letters to the editor as well as special issues.
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