通过稳定同位素标记进行代谢物注释

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2024-11-07 DOI:10.1016/j.trac.2024.118037

Yonghui Dong , Liron Feldberg , Asaph Aharoni , Uwe Heinig

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引用次数: 0

摘要

稳定同位素标记（SIL）是一种探测细胞代谢的强大工具，在基于质谱的代谢组学研究中越来越多地被用于代谢物注释。在此，我们概述了在代谢组学研究中设计和执行基于 SIL 的实验的原则和方法。此外，我们还总结了不同的 SIL 方法，并重点介绍了这些方法在代谢物注释中的最新应用实例，包括生物特征识别、退化特征注释、元素组成测定和代谢物结构阐释等。在整个过程中，我们讨论了每种 SIL 工作流程的机遇、公开挑战和潜在隐患。最后，我们总结并比较了最近开发的用于分析基于 SIL 的代谢组学数据的软件工具。

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Metabolite Annotation through Stable Isotope Labeling

Stable isotope labeling (SIL) is a powerful tool for probing cellular metabolism and has been increasingly employed for metabolite annotation in mass spectrometry-based metabolomics. Herein, we outline the principles and methods of designing and performing SIL-based experiments in metabolomics studies. Further, we summarize different SIL approaches and highlight recent examples of their use for metabolite annotation, including biological feature recognition, degenerate feature annotation, elemental composition determination and metabolite structure elucidation, across diverse biological systems. Throughout, the opportunities, open challenges, and potential pitfalls of each SIL workflow are discussed. Finally, we summarize and compare the recently developed software tools for the analysis of SIL-based metabolomics data.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.