开启微型 RNA 和环状 RNA 检测方法之旅

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2024-11-02 DOI:10.1016/j.trac.2024.118035

Zhaleh Asadi Fakhr , Xinlan Zhu , Hechen Wang , Ruiyang Ma , Ziwei Lin , Xudan Shen , Jiatong Liu , Su Zeng , Sheng Cai

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引用次数: 0

摘要

微小核糖核酸（miRNA）和环状核糖核酸（circRNA）因其独特的生物学特征而作为癌症诊断的生物标记物备受关注。众所周知，miRNA 在基因调控和组织特异性方面发挥着关键作用，但由于它们在临床样本中体积小、丰度低，因此在检测方面面临挑战。另一方面，CircRNAs 因其环状结构而具有稳定性优势，正在成为稳健的生物标记物。传统的检测方法在灵敏度和特异性方面存在局限性。等温扩增方法的最新发展有望提高检测能力。本综述重点介绍创新的引物/探针设计，这些设计提高了真实样本中 miRNA 和 circRNA 的检测效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Embarking on a journey through Micro-RNA and Circular-RNA detection methods

Micro-RNAs (miRNAs) and circular-RNAs (circRNAs) are attracting attention as biomarkers for cancer diagnosis due to their distinct biological characteristics. MiRNAs, known for their critical role in gene regulation and tissue specificity, face challenges in detection due to their small size and low abundance in clinical samples. CircRNAs, on the other hand, offer advantages in stability based on their circular structure and are emerging as robust biomarkers. Traditional detection methods have limitations in sensitivity and specificity. Recent developments in isothermal amplification methods promise improved detection capabilities. This review focuses on the innovative primer/probe designs, which enhanced the efficiency of miRNA and circRNA detection in real samples.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.