通过 PNA-DNA 杂交过程在 α-Fe2O3/Fe3O4 纳米片上构建的磁性自组装无标记电化学生物传感器,用于 APOE ε4 基因的超灵敏检测

IF 4.8 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhihao Xu , Zhixiang Lv , Huijiao Yang , Jiashuo Zhang , Zijie Sun , Dawei He , Ruijiang Liu
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引用次数: 0

摘要

研究人员开发了一种基于自组装α-Fe2O3/Fe3O4纳米片与PNA-DNA杂交过程的无标记DNA电化学检测策略,用于超灵敏检测APOE ε4基因,该基因是阿尔茨海默病(AD)最稳健的遗传风险之一。本研究采用水热煅烧还原法制备了磁性α-Fe2O3/Fe3O4异质纳米片,并在其表面负载了金纳米粒子(AuNPs)。磁性α-Fe2O3/Fe3O4@Au纳米复合材料作为信号放大基质显著增强了电化学响应,并能通过磁性自组装结合到磁性玻璃碳电极(MGCE)表面。此外,由于 PNA 与目标 DNA 具有高度的特异性和稳定的结合能力,该生物传感器不仅能准确(检测限估计为 0.147 pM)、快速地检测 APOE ε4 基因,而且表现出卓越的特异性、稳定性和再生能力。此外,该方法在实际人体血清样品中也获得了令人满意的回收率,回收率在 92.83 % 至 106.22 % 之间,相对标准偏差(RSD)在 0.25 % 至 1.85 % 之间。这些结果对探索 DNA 生物传感器技术在 APOE 基因突变诊断中的应用具有重要的参考价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Label-free electrochemical biosensor with magnetic self-assembly constructed via PNA-DNA hybridization process on α-Fe2O3/Fe3O4 nanosheets for APOE ε4 genes ultrasensitive detection

Label-free electrochemical biosensor with magnetic self-assembly constructed via PNA-DNA hybridization process on α-Fe2O3/Fe3O4 nanosheets for APOE ε4 genes ultrasensitive detection
A label-free electrochemical DNA detection strategy based on self-assembled α-Fe2O3/Fe3O4 nanosheets with PNA-DNA hybridization process was developed for ultrasensitive detection of APOE ε4 gene, one of the most robust genetic risks for Alzheimer’s Disease (AD). In this work, magnetic α-Fe2O3/Fe3O4 heterogeneous nanosheets were prepared by hydrothermal-calcined reduction method and loaded with Au nanoparticles (AuNPs) on their surfaces. The magnetic α-Fe2O3/Fe3O4@Au nanocomposites significantly enhanced the electrochemical response as a signal amplification matrix and were able to bind to the magnetic glassy carbon electrode (MGCE) surface by magnetic self-assembly. Moreover, owing to the high specificity and stable binding capacity of PNA with respect to the target DNA, the biosensor not only enabled accurate (the limit of detection was estimated to be 0.147 pM) and rapid detection of the APOE ε4 gene, but also exhibited excellent specificity, stability and regeneration capability. Additional, the satisfactory recoveries were also obtained in real samples of human serum, ranging from 92.83 % to 106.22 % with relative standard deviation (RSD) between 0.25 % and 1.85 %. The results possessed important reference value for exploring the application of DNA biosensor technology in the diagnosis of APOE gene mutation.
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来源期刊
Bioelectrochemistry
Bioelectrochemistry 生物-电化学
CiteScore
9.10
自引率
6.00%
发文量
238
审稿时长
38 days
期刊介绍: An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.
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