水三相系统一步法同时高效提取枸杞中的多种活性化合物：聚焦多糖

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2024-11-12 DOI:10.1016/j.indcrop.2024.119973

Bin Jiang, Jiaming Wang, Yu Feng, Xinhao Fu, Hongshen Yue, Dongmei Li, Chunhong Liu, Zhibiao Feng

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引用次数: 0

摘要

在过去的几年中，水相三相体系（A3PS）因其高效的分离效果而备受关注。本研究采用乙腈/甜菜碱/K2HPO4组成的水相三相体系（A3PS）作为一种新型萃取技术，同时分离了枸杞中的多糖、类胡萝卜素和多酚。该系统具有机器试剂用量少、耗材少、萃取步骤简单等特点。采用响应面法研究了乙腈、甜菜碱和 K2HPO4 浓度对萃取效率的影响。A3PS 的最佳成分为 26.2 %（重量比）乙腈、10.2 %（重量比）甜菜碱和 27.6 %（重量比）K2HPO4。在此条件下，类胡萝卜素、多酚和枸杞多糖（LBP）的提取率分别为 42.12±0.10%、88.61±0.95% 和 84.63±0.46%。所获得的枸杞多糖通过多种技术进行了表征。经证实，枸杞多糖由甘露糖、核糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖和岩藻糖组成，其比例为 4.14:0.80:4.00:4.43:22.20:39.9:12.44:3.22:8.46:0.41。枸杞多糖的分子量为 12554 Da。此外，枸杞多糖还具有优异的抗氧化活性。总之，作为一种快速发展的萃取和分离方法，A3PS 在同时分离多种物质方面具有潜在的应用价值。

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One-step simultaneous and efficient extraction of multiple active compounds from Lycium barbarum by aqueous three-phase system: Focusing on polysaccharides

In the past few years, the aqueous three-phase system (A3PS) has attracted a lot of attention due to its effectiveness in the efficient separation. Polysaccharides, carotenoids and polyphenols were separated simultaneously from Lycium barbarum by an aqueous three-phase system (A3PS) formed by acetonitrile/betaine/K₂HPO₄ as a novel extraction technique. The system is characterized by small amount of machine reagents, few consumables and simple extraction steps. The effects of acetonitrile, betaine and K₂HPO₄ concentrations on the extraction efficiency were investigated by Response Surface Methodology. The optimum composition of A3PS were 26.2 % (w/w) acetonitrile, 10.2 % (w/w) betaine and 27.6 % (w/w) K₂HPO₄. Under this condition, the extraction efficiencies of carotenoids, polyphenols, and Lycium barbarum polysaccharides (LBP) were 42.12±0.10 %, 88.61±0.95 % and 84.63±0.46 %, respectively. The obtained LBP was characterized by several technologies. The LBP was proved to consist of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose in a proportion of 4.14:0.80:4.00:4.43:22.20:39.9:12.44:3.22:8.46:0.41. The molecular weight of LBP was 12554 Da. In addition, LBP exhibited excellent antioxidant activity. In general, as a rapid developing method of extraction and separation, A3PS had potential application in the simultaneous separation of multiple substances.

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.