全面鉴定和比较分析婴儿配方粉中与蛋白质无关的 Amadori 产品：利用 LC-Q-Orbitrap-MS/MS 整合非靶向和靶向代谢组学

IF 6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

LWT - Food Science and Technology Pub Date : 2024-11-08 DOI:10.1016/j.lwt.2024.117022

Ying Dong , Heng Wang , Guodong Li , Chuyang Wang , Liqiao Han , Zemin Wan , Jun Yan , Qiaoxuan Zhang , Min Zhan , Peifeng Ke , Haibiao Lin , Xinzhong Wu , Xianzhang Huang , Pengwei Zhang

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引用次数: 0

摘要

婴儿配方奶粉容易发生马氏反应，形成早期阿莫多利产物和高级糖化终产物（AGEs）。尽管对蛋白质结合型阿莫多利产物和 AGEs 进行了大量研究，但对婴儿配方奶粉中蛋白质非结合型阿莫多利产物的研究却十分有限。本研究首次揭示了单低聚己糖和低聚己糖衍生的阿马多尔里产物表现出不同的 MS/MS 中性损失模式。利用这些特征，研究人员开发了一种靶向和非靶向相结合的策略，用于快速、全面地分析由单和低聚己糖衍生的阿莫多利产物。共鉴定出 40 种与蛋白质无关的阿马多里产物，它们是由葡萄糖、乳糖、半乳糖和 17 种氨基酸以及 3 种其他氨基酸化合物反应生成的。鉴定出了果糖基-O-磷酰乙醇胺/甘油磷酰乙醇胺和乳糖基-O-磷酰乙醇胺/甘油磷酰乙醇胺等新的阿马多尔里产物。对 5 种标准婴儿配方奶粉样品中游离的 Amadori 产品含量进行进一步比较分析后发现，它们之间存在着显著差异。不过，阿莫多利产品及其相应前体化合物的含量并没有显示出明显的相关性。这些发现为了解婴儿配方奶粉中阿马多尔里产品的复杂性和多样性提供了宝贵的见解。此外，鉴定出的 Amadori 产品有望成为区分不同制造商生产的婴儿配方奶粉的潜在标志物。

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Comprehensive identification and comparative analysis of protein-unbound Amadori products in powdered infant formulas: Integrating untargeted and targeted metabolomics with LC-Q-Orbitrap-MS/MS

Infant formula is prone to the Maillard reaction, forming early-stage Amadori products and advanced glycation end products (AGEs). Despite extensive research on protein-bound Amadori products and AGEs, studies on protein-unbound Amadori products in infant formula are limited. This study, for the first time, reveals that Amadori products derived from mono and oligohexose exhibit distinct MS/MS neutral loss patterns. Using these characteristics, a combined targeted and untargeted strategy for rapid and comprehensive analysis of mono- and oligohexose derived Amadori products was developed. A total of 40 protein-unbound Amadori products were identified, resulting from reactions between glucose, lactose, galactose, and 17 amino acids along with 3 other amino compounds. Novel Amadori products like fructosyl-O-phosphorylethanolamine/glycerophosphorylethanolamine and lactulosyl-O-phosphorylethanolamine/glycerophosphorylethanolamine were identified. Further comparative analysis of free Amadori product contents in 5 standard infant formula samples revealed significant variations. However, the levels of Amadori products and their corresponding precursor compounds showed no significant correlation. These findings offer valuable insights into the complexity and diversity of Amadori products in infant formulas. Moreover, the identified Amadori products show promise as potential markers for differentiating infant formulas from different manufacturers.

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来源期刊

LWT - Food Science and Technology 工程技术-食品科技

CiteScore

11.80

自引率

6.70%

发文量

1724

审稿时长

65 days

期刊介绍： LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.