以壳聚糖/透明质酸水凝胶为基础的伤口闭合材料的简易制备 协同负载金纳米粒子和成纤维细胞生长因子,提高糖尿病伤口愈合和护理中的抗微生物和愈合效率。

IF 3.4 3区 环境科学与生态学 Q3 CELL & TISSUE ENGINEERING
Regenerative Therapy Pub Date : 2024-11-01 eCollection Date: 2024-06-01 DOI:10.1016/j.reth.2024.10.003
Xin Liu, Shengwei Peng, Yongju Pei, Yuanyuan Huo, Yadi Zong, Jianwei Ren, Jing Zhao
{"title":"以壳聚糖/透明质酸水凝胶为基础的伤口闭合材料的简易制备 协同负载金纳米粒子和成纤维细胞生长因子,提高糖尿病伤口愈合和护理中的抗微生物和愈合效率。","authors":"Xin Liu, Shengwei Peng, Yongju Pei, Yuanyuan Huo, Yadi Zong, Jianwei Ren, Jing Zhao","doi":"10.1016/j.reth.2024.10.003","DOIUrl":null,"url":null,"abstract":"<p><p>Generally, diabetic wounds heal very slowly and inefficiently with an increasing risk of infections. Recent nanotechnology and biomaterial advances elaborate developed multi-functional hydrogels and nanoparticles offer promising solutions to accelerate wound healing for diabetic patients. This research work demonstrates to use of solvent diffusion method to develop hydrogel nanocomposites composed of chitosan (CS), hyaluronic acid (HA), gold (Au), and fibroblast growth factors (FGF). The biological analysis of nanocomposites exhibited enhanced wound healing efficiency by incorporating bioactive molecules like FGF and bioactive Au nanoparticles. <i>In vitro,</i> cell compatibility analysis (MTT assay) of prepared hydrogel nanocomposites was studied on fibroblast cell lines NIH-3T3-L1 and L929 and exhibited greater cell survival ability (>90 %), cell proliferation and migration ability, which demonstrated the suitability of nanocomposite for wound healing treatment. <i>In vitro,</i> anti-bacterial analyses established that FGF-Au@CS/HA has strong antibacterial effectiveness against gram-positive and gram-negative pathogens. The observation of the present research revealed that prepared FGF-Au@CS/HA hydrogel composites could be a suitable biomaterial for diabetic wound care, potentially improving its antibacterial and healing efficacies.</p>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"26 ","pages":"1018-1029"},"PeriodicalIF":3.4000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565426/pdf/","citationCount":"0","resultStr":"{\"title\":\"Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care.\",\"authors\":\"Xin Liu, Shengwei Peng, Yongju Pei, Yuanyuan Huo, Yadi Zong, Jianwei Ren, Jing Zhao\",\"doi\":\"10.1016/j.reth.2024.10.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Generally, diabetic wounds heal very slowly and inefficiently with an increasing risk of infections. Recent nanotechnology and biomaterial advances elaborate developed multi-functional hydrogels and nanoparticles offer promising solutions to accelerate wound healing for diabetic patients. This research work demonstrates to use of solvent diffusion method to develop hydrogel nanocomposites composed of chitosan (CS), hyaluronic acid (HA), gold (Au), and fibroblast growth factors (FGF). The biological analysis of nanocomposites exhibited enhanced wound healing efficiency by incorporating bioactive molecules like FGF and bioactive Au nanoparticles. <i>In vitro,</i> cell compatibility analysis (MTT assay) of prepared hydrogel nanocomposites was studied on fibroblast cell lines NIH-3T3-L1 and L929 and exhibited greater cell survival ability (>90 %), cell proliferation and migration ability, which demonstrated the suitability of nanocomposite for wound healing treatment. <i>In vitro,</i> anti-bacterial analyses established that FGF-Au@CS/HA has strong antibacterial effectiveness against gram-positive and gram-negative pathogens. The observation of the present research revealed that prepared FGF-Au@CS/HA hydrogel composites could be a suitable biomaterial for diabetic wound care, potentially improving its antibacterial and healing efficacies.</p>\",\"PeriodicalId\":20895,\"journal\":{\"name\":\"Regenerative Therapy\",\"volume\":\"26 \",\"pages\":\"1018-1029\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565426/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Regenerative Therapy\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1016/j.reth.2024.10.003\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"CELL & TISSUE ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regenerative Therapy","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.reth.2024.10.003","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"CELL & TISSUE ENGINEERING","Score":null,"Total":0}
引用次数: 0

摘要

一般来说,糖尿病伤口愈合非常缓慢且效率低下,感染风险不断增加。最近纳米技术和生物材料的进步精心研制的多功能水凝胶和纳米粒子为加速糖尿病患者的伤口愈合提供了有希望的解决方案。这项研究工作展示了如何利用溶剂扩散法开发由壳聚糖(CS)、透明质酸(HA)、金(Au)和成纤维细胞生长因子(FGF)组成的水凝胶纳米复合材料。纳米复合材料的生物学分析表明,加入 FGF 等生物活性分子和生物活性金纳米粒子后,伤口愈合效率有所提高。在体外,对制备的水凝胶纳米复合材料的成纤维细胞系 NIH-3T3-L1 和 L929 进行了细胞相容性分析(MTT 试验),结果表明纳米复合材料具有更强的细胞存活能力(大于 90%)、细胞增殖和迁移能力,这表明纳米复合材料适合用于伤口愈合治疗。体外抗菌分析表明,FGF-Au@CS/HA 对革兰氏阳性和革兰氏阴性病原体具有很强的抗菌效果。本研究的观察结果表明,制备的 FGF-Au@CS/HA 水凝胶复合材料可作为一种适用于糖尿病伤口护理的生物材料,并有可能提高其抗菌和愈合功效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Facile fabrication of chitosan/hyaluronic acid hydrogel-based wound closure material Co-loaded with gold nanoparticles and fibroblast growth factor to improve anti-microbial and healing efficiency in diabetic wound healing and nursing care.

Generally, diabetic wounds heal very slowly and inefficiently with an increasing risk of infections. Recent nanotechnology and biomaterial advances elaborate developed multi-functional hydrogels and nanoparticles offer promising solutions to accelerate wound healing for diabetic patients. This research work demonstrates to use of solvent diffusion method to develop hydrogel nanocomposites composed of chitosan (CS), hyaluronic acid (HA), gold (Au), and fibroblast growth factors (FGF). The biological analysis of nanocomposites exhibited enhanced wound healing efficiency by incorporating bioactive molecules like FGF and bioactive Au nanoparticles. In vitro, cell compatibility analysis (MTT assay) of prepared hydrogel nanocomposites was studied on fibroblast cell lines NIH-3T3-L1 and L929 and exhibited greater cell survival ability (>90 %), cell proliferation and migration ability, which demonstrated the suitability of nanocomposite for wound healing treatment. In vitro, anti-bacterial analyses established that FGF-Au@CS/HA has strong antibacterial effectiveness against gram-positive and gram-negative pathogens. The observation of the present research revealed that prepared FGF-Au@CS/HA hydrogel composites could be a suitable biomaterial for diabetic wound care, potentially improving its antibacterial and healing efficacies.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Regenerative Therapy
Regenerative Therapy Engineering-Biomedical Engineering
CiteScore
6.00
自引率
2.30%
发文量
106
审稿时长
49 days
期刊介绍: Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信