Diesel-degradation by indigenous bacteria of petroleum-contaminated soils.

Relying on native microorganisms is crucial for bioremediating petroleum-contaminated soils within this oil field. This study aimed to isolate native bacteria and investigate their ability to degrade petroleum hydrocarbons in contaminated soils. Flame ionization detector gas chromatography analyzed the capacity of Indigenous bacterial isolates to break down diesel fuel in an aquatic environment. Soil samples were collected from the Naft-Shahr area. Initially, 126 bacterial isolates were obtained from these soils, of which only 48 species could grow on a diesel-containing medium. Further analysis identified the top 8 isolates with high diesel removal potential. Results showed that the diesel removal percentage ranged from 26 to 76% at an initial diesel concentration of 3.7 g. L - ¹ after 48 h, without adding any supplementary surface-active agent. Four top isolates were selected based on their degradation activity, removal yield, and biodegradation rate and were identified using 16S rRNA gene sequencing and phylogenetic analysis. Sequence alignment revealed that isolates B11Pet, B19Pet, B27Pet, and B48Pet belong to Staphylococcus gallinarum, Paenarthrobacter nitroguajacolicus, Arthrobacter citreus, and Bacillus thuringiensis, respectively. Among these, Bacillus thuringiensis (B48Pet), with a specific growth rate of 0.211 h⁻¹, could uniformly remove all diesel hydrocarbon fractions at 58.81 mg. L⁻¹. h⁻¹. This strain, alone or in consortia, represents a promising strategy for the bioremediation of petroleum-contaminated soils.