一种来自 Gracilariadomingensis 的硫酸化多糖的结构特征及潜在的抗炎和抗痛觉作用。

IF 2.4 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Rafael da Silva Prudêncio, Antonio Kleiton de Sousa, Denise Mayara Melo Silva, Jayro Dos Santos Ferreira, Danyela Maria Leal Rocha, Viviane Pinheiro Alves de Almeida, João Janilson da Silva Sousa, Stefany Guimarães Sousa, Tino Marcos Lino da Silva, André Dos Santos Carvalho, José Simião da Cruz Júnior, Even Herlany Pereira Alves, Daniel Fernando Pereira Vasconcelos, Roosevelt D S Bezerra, Flaviane de França Dourado, Aline Lima de Oliveira, Wanessa Feliz Cabral, José Roberto de Souza de Almeida Leite, Durcilene Alves da Silva, Elias Borges do Nascimento Junior, Tarcísio Vieira de Brito, Diva de Aguiar Magalhães, André Luiz Dos Reis Barbosa
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引用次数: 0

摘要

海藻是硫酸化多糖(SPs)的天然来源,硫酸化多糖是一种生物聚合物,具有显著的药理特性,包括能够减轻水肿、细胞因子、细胞迁移和疼痛等炎症过程的生物作用。我们的研究结果证实,从Gracilaria domingensis获得的SPs(SP-GD)是一种琼脂糖,主要由β-d-半乳糖6-硫酸酯和3,6-脱水-α-半乳糖残基组成。具体来说,10 毫克/千克剂量的 SP-GD 能有效减轻角叉菜胶或组胺、5-羟色胺、缓激肽、48/80 和前列腺素 E2 引起的爪水肿。SP-GD (10 毫克/千克)还能减少角叉菜胶诱导的腹膜炎中的中性粒细胞迁移和髓过氧化物酶的活性,并能保护谷胱甘肽浓度和降低动物腹腔液中丙二醛的含量。此外,它还在醋酸诱导的腹部蠕动试验和福尔马林诱导的爪舔试验中显示出抗痛作用。因此,根据所获得的结果,我们可以推断剂量为 10 毫克/千克的多明戈萃取物具有抗炎作用,可减少中性粒细胞的迁移,调节血管活性介质的活性,还具有抗痛觉作用,至少部分作用是通过依赖于炎症介质负向调节的外周机制产生的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural characterization of a sulfated polysaccharide from Gracilariadomingensis and potential anti-inflammatory and antinociceptive effects.

Seaweeds are natural sources of sulfated polysaccharides (SPs), biopolymers with remarkable pharmacological properties, including biological actions capable of attenuating components of the inflammatory process such as edema, cytokines, cell migration and pain. Our results confirm that SPs obtained from Gracilaria domingensis (SP-GD) are agarans, primarily composed of residues of β-d-galactopyranose 6-sulfate and 3,6-anhydro-α-l-galactopyranose. Specifically, SP-GD at a dose of 10 mg/kg was effective in significantly reducing paw edema induced by carrageenan or histamine, serotonin, bradykinin, 48/80 and prostaglandin E2. SP-GD (10 mg/kg) was also able to reduce neutrophil migration and the activity of the myeloperoxidase enzyme in carrageenan-induced peritonitis, as well as conserve glutathione concentration and reduce malondialdehyde levels in the animals' peritoneal fluid. Furthermore, it showed antinociceptive action in the abdominal writhing test induced by acetic acid and in the paw licking test induced by formalin. Thus, the results obtained allow us to infer that SPs extracted from G. domingensis at a dose of 10 mg/kg have anti-inflammatory effects by reducing neutrophil migration and modulating the activity of vasoactive mediators and antinociceptive effects by acting, at least in part, through a peripheral mechanism dependent on the negative modulation of inflammatory mediators.

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来源期刊
Carbohydrate Research
Carbohydrate Research 化学-生化与分子生物学
CiteScore
5.00
自引率
3.20%
发文量
183
审稿时长
3.6 weeks
期刊介绍: Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
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