从 Hylocereus undatus 果皮废弃物中生物合成银纳米粒子：探索抑制表皮生长因子受体用于宫颈癌和乳腺癌靶向治疗的方法

IF 3.4 Q2 PHARMACOLOGY & PHARMACY

Future Journal of Pharmaceutical Sciences Pub Date : 2024-11-15 DOI:10.1186/s43094-024-00737-8

Kushala Reddy, Preeti Salve

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引用次数: 0

摘要

背景癌症是导致全球死亡的主要原因之一，其中乳腺癌和宫颈癌在女性中最为常见。每年新确诊的乳腺癌病例超过 100,000 例，宫颈癌病例超过 510,000 例。本研究旨在开发和评估一种环保、低成本的方法，利用火龙果果皮提取物合成银纳米粒子，以研究其抗癌活性。结果通过绿色合成技术成功开发出了负载火龙果果皮提取物的银纳米粒子，并通过紫外可见光谱进行了优化。纳米颗粒的平均粒径为 71.66 nm，多分散指数为 0.3754，zeta 电位为 -38.52，呈球形，含银量为 79.5%。它们的最大吸光度为 448 纳米。通过 MTT（3-(4, 5-二甲基噻唑基-2)-2, 5-二苯基溴化四氮唑）测定法评估了体外抗癌活性，合成的纳米颗粒对 Hela 和 MDA MB 231 细胞株的 IC50 值分别为 23.51 µg/ml 和 23.66 µg/ml。细胞相容性研究显示，在 L929 小鼠成纤维细胞中细胞存活率高（≥ 95%），表明毒性低。包括网络药理学和分子对接在内的硅学分析发现，山奈酚和槲皮素是关键的抗癌化合物，而表皮生长因子受体（EGFR）（PDB ID：IM17）是最重要的蛋白质靶点。使用 Schrodinger 软件的 Glide 模块进行的对接研究显示，与标准药物厄洛替尼相比，山奈酚和槲皮素与表皮生长因子受体的结合亲和力更高，其中 MET 769 是关键的结合位点。

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Biogenic synthesis of silver nanoparticles from Hylocereus undatus peel waste: exploring EGFR inhibition for targeted therapy of cervical and breast carcinomas

Background

Cancer is one of the leading causes of death worldwide, with breast and cervical cancers being the most common among women. Over 100,000 new cases of breast cancer and 510,000 new cases of cervical cancer are diagnosed annually. This study aimed to develop and evaluate an eco-friendly, low-cost method to synthesize silver nanoparticles using Hylocereus undatus (dragon fruit) peel extract for their anticancer activity.

Results

Silver nanoparticles loaded with Hylocereus undatus fruit peel extract were successfully developed by a green synthesis technique and were optimized by UV–vis spectroscopy. The nanoparticles had an average size of 71.66 nm, a polydispersity index of 0.3754, and a zeta potential of − 38.52, with a spherical shape and 79.5% silver content. Their maximum absorbance was at 448 nm. Further, in vitro anticancer activity via MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay was evaluated and the synthesized nanoparticles displayed IC₅₀ values at 23.51 µg/ml and 23.66 µg/ml against Hela and MDA MB 231 cell lines, respectively. Cytocompatibility studies showed high cell viability (≥ 95%) in L929 mouse fibroblast cells, indicating low toxicity. In silico analyses, including network pharmacology and molecular docking, identified kaempferol and quercetin as key anticancer compounds, with epidermal growth factor receptor (EGFR) (PDB ID: IM17) being the most significant protein target. Docking studies performed by using the Glide module of Schrodinger’s software displayed that kaempferol and quercetin had higher binding affinities for EGFR as compared to the standard drug erlotinib, with MET 769 being a crucial binding site.

Conclusion

Thus, the outcomes suggest that synthesized silver nanoparticles loaded with Hylocereus undatus fruit peel extract could be a potential and promising drug carrier aiding in cancer treatment.

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来源期刊

Future Journal of Pharmaceutical Sciences PHARMACOLOGY & PHARMACY-

自引率

0.00%

发文量

审稿时长

23 weeks

期刊介绍： Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.