N Izwan-Anas, Z Ya'cob, V L Low, E Y Lourdes, B-T Teoh, M S Mansor, R Ramli, P Pramual, P H Adler, H Takaoka, S AbuBakar
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引用次数: 0
摘要
经证实，Simulium asakoae Takaoka 和 Davies 可叮咬人类，并被认为是白细胞虫属和锥虫属血液原生动物寄生虫以及泰国一种未知丝虫寄生虫的传播媒介。然而，在马来西亚，它对人类的吸引力仍未得到研究。最近，我们在马来西亚彭亨州收集到了 27 只黑蝇，其中 25 只是用二氧化碳诱饵捕获的，另外 2 只是在诱捕活动中从人类身上收集到的。所有标本经形态鉴定均为 S. asakoae。在人类身上捕获的两个标本的细胞色素 c 氧化酶 I 序列与 NCBI GenBank 中的 S. asakoae 相似度达 100%，证实了它们的形态特征。使用 HAL-HARTM 试剂盒进行的血餐分析没有发现家畜或野生动物的 DNA。不过，在细胞色素 b 基因扩增试验中，从一只被吞食的苍蝇身上扩增出了人类 DNA，这首次证明了 S. asakoae 在马来西亚以血为食。
First blood-meal record of Simulium asakoae (Diptera: Simuliidae) in Malaysia, with notes on its distribution in Asia and status as a potential vector.
Simulium asakoae Takaoka and Davies has been confirmed to bite humans and has been incriminated as a vector of blood protozoan parasites of the genera Leucocytozoon and Trypanosoma, as well as an unknown filarial parasite in Thailand. However, its attraction to humans has remained uninvestigated in Malaysia. Recently, 27 black flies were collected in Pahang, Malaysia, of which 25 were captured in CO2-baited Malaise traps and two were collected from humans during trapping activity. All specimens were morphologically identified as S. asakoae. Cytochrome c oxidase I sequences of the two specimens caught on humans showed 100% similarity with those of S. asakoae in the NCBI GenBank, confirming their morphological identity. Blood-meal analysis using a HAL·HARTM kit did not show the presence of domestic or wild animal DNA. However, human DNA was amplified from one engorged fly in the cytochrome b gene amplification assay, providing the first evidence of blood-feeding by S. asakoae in Malaysia.
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