HvMORF8赋予大麦耐旱性的转录组和分子证据

IF 6.1 2区 生物学 Q1 PLANT SCIENCES
Shou-Heng Shi , Muhammad Zeeshan , Wu-Nian Shan , Cheng-Wei Qiu , Zhong-Hua Chen , Feibo Wu
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引用次数: 0

摘要

干旱是全球最具破坏性的非生物胁迫之一,严重限制了作物产量。西藏野生青稞是改良作物包括耐旱性的有用基因宝库。在这里,我们检测到了基因表达对干旱胁迫反应的大规模变化,西藏野生青稞基因型 XZ5(耐旱)、XZ54(对干旱敏感)和 cv. Tadmor(抗旱)之间存在巨大差异。Tadmor(耐旱)。干旱胁迫导致 XZ5 中涉及转录、代谢、蛋白质合成、胁迫防御、转运和信号转导的 142 个基因上调,但这些基因在 XZ54 和 Tadmor 中下调或不变。我们发现了一种新的多细胞器RNA编辑因子8(HvMORF8),并对其进行了功能表征。在干旱胁迫下,该因子在XZ5中上调,但在XZ54和Tadmor中没有变化。系统进化分析表明,HvMORF8的直向同源物可以追溯到最接近的裸子植物物种,如苏铁(Cycas micholitzii),这意味着MORF8的进化起源可能来自早期种子植物的共同祖先。病毒诱导的 HvMORF8 在 XZ5 中沉默会导致对干旱胁迫的超敏反应,这表明它是大麦耐旱性的正调控因子。对 BSMV:HvMORF8 和对照植株的 RNA 测序发现,沉默 HvMORF8 会抑制涉及渗透溶质运输、细胞壁修饰和抗氧化剂的基因,从而导致干旱胁迫下水分代谢紊乱和活性氧(ROS)过度积累。因此,我们从转录组水平提出了HvMORF8介导的XZ5抗旱调控机制,为研究HvMORF8质粒RNA编辑功能对大麦抗旱性的遗传基础提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptome and molecular evidence of HvMORF8 conferring drought-tolerance in barley
Drought is one of the most devastating abiotic stresses worldwide, which severely limits crop yield. Tibetan wild barley is a treasure trove of useful genes for crop improvement including drought tolerance. Here, we detected large-scale changes of gene expression in response to drought stress with a substantial difference among contrasting Tibetan barley genotypes XZ5 (drought-tolerant), XZ54 (drought-sensitive) and cv. Tadmor (drought-tolerant). Drought stress led to upregulations of 142 genes involved in transcription, metabolism, protein synthesis, stress defense, transport and signal transduction in XZ5, but those genes were down-regulated or unchanged in XZ54 and Tadmor. We identified and functionally characterized a novel multiple organellar RNA editing factors 8 (HvMORF8), which was up-regulated by drought stress in XZ5, but unchanged in XZ54 and Tadmor under drought stress. Phylogenetic analysis showed that orthologues of HvMORF8 can be traced back to the closest gymnosperm species such as Cycas micholitzii, implicating a potential evolutionary origin for MORF8 from a common ancestor in early seed plants. Virus-induced HvMORF8 silencing in XZ5 led to hypersensitivity to drought stress, demonstrating it is a positive regulator of drought tolerance in barley. RNA sequencing of BSMV:HvMORF8 and control plants reveals that silencing of HvMORF8 suppresses genes involved in osmolytes transport, cell wall modification and antioxidants, resulting in water metabolism disorder and overaccumulation of reactive oxygen species (ROS) under drought stress. Therefore, we propose HvMORF8-mediated regulatory drought tolerance mechanisms at transcriptomic level in XZ5, providing new insight into the genetic basis of plastid RNA editing function of HvMORF8 for barley drought tolerance.
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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