利用罗望子的种子渗出物增强百部皂苷的合成。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-10 DOI:10.1016/j.plaphy.2024.109287

Titir Guha , Ekta Bhattacharya , Madhurima Dutta , Anisha Dutta , Moumita Dandapat , Rahul Bose , Suparna Mandal Biswas

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引用次数: 0

摘要

植物的不同部位会通过浸出、渗出、挥发等方式释放出多种等位化学物质，根据目标植物种类的不同，这些化学物质可产生刺激或抑制作用。有关通过种子渗出物进行等位异化作用的详细报道很少。由于已知罗望子种子渗出物（TSE）对莴苣、萝卜和芝麻具有生长刺激作用，在本研究中，我们评估了它在调节一种被过度开发的药用植物百部（Bacopa monnieri (L.) Pennel）次生代谢中的作用。与制药业日益增长的需求相比，百服宁的生物合成率相当低。目前，研究的目标是通过利用外部胁迫因素来提高这种次生代谢物在植物体内的生物合成。目前，用 1:16、1:8、1:4、1:3 和 1:2（种子重量：水）的 TSE 处理 7 天大的猴面包树幼苗。在 1:4 （种子重量：水）TSE 处理组中，次生代谢物含量的上调幅度最大。这种 TSE 处理还提高了 H2O2 和水杨酸的产量，导致负责巴豆苷生物合成的 MVA 途径相关基因（BmAACT、BmHMGR、BmMDD、BmSQS 和 BmBAS）上调，TSE 处理组的巴豆苷含量比对照组高 1.7 倍。TSE的LC-HRMS分析证实了生物碱（羽扇豆碱）、酚（绿原酸）和有机酸（粘液酸）的存在，这些物质被确定为潜在的等位化学物质，负责调节猴面包树的次生代谢。因此，本研究强调了提高植物体内巴豆苷产量的可持续方法。

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Enhanced bacoside synthesis in Bacopa monnieri plants using seed exudates from Tamarindus indica

Diverse allelochemicals are released from different plant parts via leaching, exudation, volatilization, etc., which can induce either stimulatory or inhibitory effects depending on the target plant species. Very few reports provide details about allelopathic interaction through seed exudates. Since Tamarindus indica L. seed exudate (TSE) has been known to exhibit growth stimulatory effect on lettuce, radish, and sesame, in the present study we have evaluated its role in regulating the secondary metabolism of an over-exploited medicinal herb, Bacopa monnieri (L.) Pennel. The bacoside biosynthesis rate of B. monnieri is quite low in comparison to its increasingly high demands in the pharmaceutical industry. Currently, researches are aimed towards enhancing the biosynthesis of this secondary metabolite in planta by utilizing external stress factors. Presently, 7-day-old B. monnieri seedlings were treated with 1:16, 1:8, 1:4, 1:3, and 1:2 (seed weight: water) TSE. Maximum upregulation of secondary metabolite contents was found in the 1:4 (seed weight: water) TSE treatment set. This TSE treatment also enhanced H₂O₂ and salicylic acid production leading to the upregulation of the genes related to the MVA pathway (BmAACT, BmHMGR, BmMDD, BmSQS, and BmBAS) which are responsible for bacoside biosynthesis and 1.7-fold higher bacoside level was found in TSE treated set compared to control. LC-HRMS analysis of TSE confirmed the presence of alkaloid (lupanine), phenol (chlorogenic acid), and organic acid (mucic acid), which are identified as potential allelochemicals responsible for modulating the secondary metabolism of B. monnieri. Thus, this study highlights a sustainable approach towards enhancing bacoside production in planta.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.