Yuanhong Wang, Junna Wang, You Zhao, Yun Liu, Miao Zhang, Xiaoying Deng, Jie Zhu, Guoxin Li, Guangqing Liu
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引用次数: 0
摘要
猫冠状病毒（FCoV）是一种有包膜的正义 RNA 病毒，在猫科动物中广泛传播，可引起一种致命的严重疾病--猫传染性腹膜炎（FIP）。根据抗原和基因组成的不同，FCoV 分为 FCoV I 和 FCoV II 两种基因型。在这项研究中，我们分离并鉴定了一种名为 HL2019 的 FCoV I 型菌株。基于 HL2019 的完整基因组，系统进化分析表明，HL2019 株与人类冠状病毒 229E（HCoV 229E）和 HCoV NL63 更接近，形成 FCoV I 群，而 FCoV I 株与 FCoV II 株关系较远。用 RDP4 和 Simplot 软件分析表明，HL2019 病毒是由 FCoV I 中国/ZJU1709 株和 FCoV I 荷兰/UU16 株重组而来。此外，还在 9-12 个月大的猫身上评估了 HL2019 的致病性。三只受到挑战的猫中有两只出现了严重的临床症状，并在挑战后 28 天（dpc）死亡。实时聚合酶链反应（PCR）分析表明，HL2019 具有广泛的组织滋养性，尤其是在十二指肠中，病毒载量高达 104 拷贝/毫克。总之，我们的数据表明，我们成功分离出了一株对猫具有高致病性的 FCoV I，命名为 HL2019。
Isolation and Genomic Characteristics of a Novel Pathogenicity Type I Feline Coronavirus in Mainland China
Feline coronavirus (FCoV) is an enveloped, positive-sense RNA virus, which is widespread among feline populations, and can cause a fatal serious disease called feline infectious peritonitis (FIP). According to the differences of antigen and genetic composition, FCoV consists of two genotypes, FCoV I and FCoV II. In this study, we have isolated and identified a FCoV I strain named HL2019. Based on the complete genome of HL2019, phylogenetic analysis showed that HL2019 strain formed in the cluster FCoV I which is more closed to human coronavirus 229E (HCoV 229E) and HCoV NL63, while the FCoV I stains is distantly related to FCoV II strains. Analyzing with RDP4 and Simplot software showed that the virus HL2019 is recombinant by the FCoV I China/ZJU1709 and FCoV I Netherlands/UU16 strains. Furthermore, the pathogenicity of HL2019 was evaluated in 9–12-month-old cats. Two of three challenged cats developed serious clinical signs and died at 28-day postchallenge (dpc). Real-time polymerase chain reaction (PCR) analysis showed that HL2019 has broad tissue tropism, especially in the duodenum with viral load up to 104 copies/mg. In summary, our data show that we have successfully isolated a strain of FCoV I named HL2019 that is highly pathogenic to cats.
期刊介绍：
Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions):
Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread.
Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope.
Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies.
Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies).
Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.
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