{"title":"芽孢杆菌属在微囊藻毒素生物降解中的作用：对清除热带淡水中蓝藻毒素的影响","authors":"Madhuni Madhushika Wijesooriya, Kanaji Masakorala, Widana Gamage Shirani Manel Kumari","doi":"10.1002/aws2.70005","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <p>The proliferation of harmful cyanobacterial blooms poses a threat to the ecosystem and human health. Microcystins (MCs) are the most frequently detected cyanotoxin released by cyanobacteria, including <i>Microcystis</i> sp. Our study aimed to isolate potential MC-degrading bacteria in Sri Lankan freshwater and determine their possible MC-degradation pathways. Twenty-two morphologically distinct bacterial strains from 13 freshwater bodies were isolated following the enrichment assay. MC-biodegradation assays further confirmed the MC-degradation potential of 21 strains, with <i>Bacillus</i> being the predominant genus. The strain <i>Bacillus altitudinis</i> BL1, isolated from Beira Lake, showed the highest efficiency in <i>Microcystis</i> sp. cell lysis (80%) and MC-degradation (87%). PCR assay results confirmed the absence of <i>mlr</i>ABCD genes in all strains, indicating the presence of uncharacterized alternative MC-degradation mechanisms that require further exploration. The dual functions of <i>Microcystis</i> sp. cell lysis and MC-degradation in 21 strains support developing efficient bacteria-mediated strategies to remediate microcystins and eradicate <i>Microcystis</i>-blooms in tropical freshwaters.</p>\n </section>\n </div>","PeriodicalId":101301,"journal":{"name":"AWWA water science","volume":"6 6","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The role of genus Bacillus in biodegradation of microcystins: Implications for the removal of cyanotoxins from tropical freshwaters\",\"authors\":\"Madhuni Madhushika Wijesooriya, Kanaji Masakorala, Widana Gamage Shirani Manel Kumari\",\"doi\":\"10.1002/aws2.70005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <p>The proliferation of harmful cyanobacterial blooms poses a threat to the ecosystem and human health. Microcystins (MCs) are the most frequently detected cyanotoxin released by cyanobacteria, including <i>Microcystis</i> sp. Our study aimed to isolate potential MC-degrading bacteria in Sri Lankan freshwater and determine their possible MC-degradation pathways. Twenty-two morphologically distinct bacterial strains from 13 freshwater bodies were isolated following the enrichment assay. MC-biodegradation assays further confirmed the MC-degradation potential of 21 strains, with <i>Bacillus</i> being the predominant genus. The strain <i>Bacillus altitudinis</i> BL1, isolated from Beira Lake, showed the highest efficiency in <i>Microcystis</i> sp. cell lysis (80%) and MC-degradation (87%). PCR assay results confirmed the absence of <i>mlr</i>ABCD genes in all strains, indicating the presence of uncharacterized alternative MC-degradation mechanisms that require further exploration. The dual functions of <i>Microcystis</i> sp. cell lysis and MC-degradation in 21 strains support developing efficient bacteria-mediated strategies to remediate microcystins and eradicate <i>Microcystis</i>-blooms in tropical freshwaters.</p>\\n </section>\\n </div>\",\"PeriodicalId\":101301,\"journal\":{\"name\":\"AWWA water science\",\"volume\":\"6 6\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-11-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"AWWA water science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/aws2.70005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"AWWA water science","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/aws2.70005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
有害蓝藻水华的扩散对生态系统和人类健康构成威胁。微囊藻毒素（MC）是蓝藻（包括微囊藻）释放的最常见的蓝藻毒素。通过富集试验，我们从 13 个淡水水体中分离出了 22 株形态各异的细菌。二氯甲烷生物降解试验进一步证实了 21 株菌株的二氯甲烷降解潜力，其中芽孢杆菌是主要的菌属。从贝拉湖分离出的菌株 Bacillus altitudinis BL1 对微囊藻细胞裂解（80%）和 MC 降解（87%）的效率最高。PCR 检测结果证实，所有菌株中都没有 mlrABCD 基因，这表明存在尚未定性的替代 MC 降解机制，需要进一步探索。21 株微囊藻细胞裂解和 MC 降解的双重功能支持开发高效的细菌介导策略，以修复微囊藻毒素并根除热带淡水中的微囊藻。
The role of genus Bacillus in biodegradation of microcystins: Implications for the removal of cyanotoxins from tropical freshwaters
The proliferation of harmful cyanobacterial blooms poses a threat to the ecosystem and human health. Microcystins (MCs) are the most frequently detected cyanotoxin released by cyanobacteria, including Microcystis sp. Our study aimed to isolate potential MC-degrading bacteria in Sri Lankan freshwater and determine their possible MC-degradation pathways. Twenty-two morphologically distinct bacterial strains from 13 freshwater bodies were isolated following the enrichment assay. MC-biodegradation assays further confirmed the MC-degradation potential of 21 strains, with Bacillus being the predominant genus. The strain Bacillus altitudinis BL1, isolated from Beira Lake, showed the highest efficiency in Microcystis sp. cell lysis (80%) and MC-degradation (87%). PCR assay results confirmed the absence of mlrABCD genes in all strains, indicating the presence of uncharacterized alternative MC-degradation mechanisms that require further exploration. The dual functions of Microcystis sp. cell lysis and MC-degradation in 21 strains support developing efficient bacteria-mediated strategies to remediate microcystins and eradicate Microcystis-blooms in tropical freshwaters.
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