壳聚糖包裹的硒纳米粒子通过协同调节NF-κB和Nrf2信号通路以及调控Bcl-2和Caspase-3的表达,减轻了CCl4诱导的肝毒性:多元回归分析综合研究

IF 3.6 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Alaa abouelazayem Mrwad , Shaymaa E. El-Shafey , Noha Mohamed Said
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引用次数: 0

摘要

背景:以纳米形式(Se-NPs)递送硒是治疗各种氧化应激引起的疾病的一种很有前景的方法:本研究旨在探讨硒纳米粒子单独(Se-NPs)或与壳聚糖封装在一起(Se-CS-NPs)对大鼠由 CCl4 引起的毒性的影响:将 80 只白化大鼠平均分为 8 组。第一组为安慰剂组。第二组为阳性对照组,第三组和第四组分别口服(Se-NPs 5 mg/Kg)和(Se-CS-NPs 225 mg/Kg)。第五组和第六组为保护组,同时给予 Se-NPs 或 Se-CS-NPs。第七组和第八组为治疗组,在停止注射 CCl4 4 周后接受 Se-NPs 或 Se-CS-NPs 治疗:结果表明,保护组和治疗组的 Caspase-3 基因表达增加,Bcl-2、Nrf2 和 AFP 基因表达下降。组织病理学和免疫组织化学研究表明,硒纳米颗粒单独或与壳聚糖包被在降低纤维化标志物胶原 I 阳性反应方面的作用。此外,使用纳米硒粒子还能有效保护肝脏结构及其正常成分。用壳聚糖包裹硒纳米粒子时,没有观察到额外的益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chitosan-encapsulated selenium nanoparticles alleviate CCl4 induced hepatotoxicity through synergistically modulating NF-κB and Nrf2 signaling pathways and regulating Bcl-2 and Caspase-3 expression: A comprehensive study with multiple regression analysis

Background

The delivery of selenium in a nano-form (Se-NPs) is a promising modality of treatment for various oxidative stress-induced diseases.

Objective

This study aims to investigate the conceivable effects of selenium nanoparticles either alone (Se-NPs) or encapsulated with chitosan (Se-CS-NPs) on toxicity induced by CCl4 in rats.

Methods

Eighty albino rats were divided equally into eight groups. The first group was the placebo. The second group was a positive control, while the third and the fourth groups got orally (Se-NPs 5 mg/Kg) and (Se-CS-NPs 225 mg/Kg) respectively. The fifth and sixth groups were protective groups in which Se-NPs or Se-CS-NPs were given simultaneously. The seventh and eighth groups were therapeutic as they received either Se-NPs or Se-CS-NPs after stopping the CCl4 injection for 4 weeks more.

Results

Our results showed that the protective and therapeutic groups showed an increase in caspase-3 gene expression with a decline in the expression of Bcl-2, Nrf2, and AFP genes. Histopathological and immunohistochemical investigations showed the role of selenium nanoparticles either alone or coated with chitosan in decreasing fibrotic marker collagen I positive reaction

Conclusion

Selenium nanoparticles showed an excellent effect in counteracting the toxic effect of carbon tetrachloride on liver functions, inflammation reactions, and apoptosis process. Moreover, using selenium nanoparticles has a strong role in preserving the liver architecture with its normal constituents. No additional benefit was observed when the selenium nanoparticles were encapsulated with chitosan.
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来源期刊
CiteScore
6.60
自引率
2.90%
发文量
202
审稿时长
85 days
期刊介绍: The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.
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