空间转录组学揭示了成年小鼠口腔黏膜及其干细胞龛位部位特异性的分子线索。

IF 5.9 2区 医学 Q1 CELL & TISSUE ENGINEERING
Stem Cell Reports Pub Date : 2024-12-10 Epub Date: 2024-11-27 DOI:10.1016/j.stemcr.2024.10.007
Anna C Seubert, Marion Krafft, Sarah Bopp, Moutaz Helal, Pranjali Bhandare, Elmar Wolf, Anna Alemany, Angela Riedel, Kai Kretzschmar
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引用次数: 0

摘要

口腔是一个多功能器官,由结构上异质的粘膜组织组成,其特征仍然不甚明了。口腔黏膜组织高度分层,并沿上皮-固有膜轴分段。在这里,我们对成年小鼠的舌头、脸颊和上颚进行了空间转录组学(tomo-seq)研究,以了解维持口腔黏膜部位的线索。我们定义了不同口腔部位独特和共享细胞龛和分化程序的分子标记。通过比较方法,我们发现成纤维细胞生长因子(FGF)通路成分是口腔上皮干细胞的潜在干细胞龛因子。利用类器官形成效率测定法,我们验证了三种FGF配体(FGF1、FGF7和FGF10)是舌背和舌腹部位的特异性龛因子。我们的数据集对口腔主要部位的基因进行了空间解析,为揭示口腔黏膜及其干细胞龛位的成人稳态分子机制提供了全面的资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spatial transcriptomics reveals molecular cues underlying the site specificity of the adult mouse oral mucosa and its stem cell niches.

The oral cavity is a multifunctional organ composed of structurally heterogeneous mucosal tissues that remain poorly characterized. Oral mucosal tissues are highly stratified and segmented along an epithelial-lamina propria axis. Here, we performed spatial transcriptomics (tomo-seq) on the tongue, cheeks, and palate of the adult mouse to understand the cues that maintain the oral mucosal sites. We define molecular markers of unique and shared cellular niches and differentiation programs across oral sites. Using a comparative approach, we identify fibroblast growth factor (FGF) pathway components as potential stem cell niche factors for oral epithelial stem cells. Using organoid-forming efficiency assays, we validated three FGF ligands (FGF1, FGF7, and FGF10) as site-specific niche factors in the dorsal and ventral tongue. Our dataset of the spatially resolved genes across major oral sites represents a comprehensive resource for unraveling the molecular mechanisms underlying the adult homeostasis of the oral mucosa and its stem cell niches.

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来源期刊
Stem Cell Reports
Stem Cell Reports CELL & TISSUE ENGINEERING-CELL BIOLOGY
CiteScore
10.50
自引率
1.70%
发文量
200
审稿时长
28 weeks
期刊介绍: Stem Cell Reports publishes high-quality, peer-reviewed research presenting conceptual or practical advances across the breadth of stem cell research and its applications to medicine. Our particular focus on shorter, single-point articles, timely publication, strong editorial decision-making and scientific input by leaders in the field and a "scoop protection" mechanism are reasons to submit your best papers.
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