{"title":"基于碳点-氧化石墨烯的发光纳米传感器用于检测人体尿液中的肌酐。","authors":"Poornima Bhatt, Deepak Kukkar, Ashok Kumar Yadav","doi":"10.1007/s00604-024-06838-8","DOIUrl":null,"url":null,"abstract":"<div><p>A fluorescence (FL)-based nanosensor has been devised for creatinine (CR) detection in human urine specimens. The proposed nanosensor utilized a nanocomposite (NC) of carbon dots (CDs) and graphene oxide (GO). The formation of CDs/GO NC reduced the CD FL emission (<i>λ</i><sub>excitation</sub> = 390 nm, <i>λ</i><sub>emission</sub> = 461 nm) by ~ 75%. With the introduction of CR to the NC, the CD emission intensity was reinstated by approximately 70%. The linear detection range for CR was 10<sup>−5</sup> to 0.1 mg dL<sup>−1</sup> (<i>R</i><sup>2</sup> = 0.998), with a limit of detection of 4.3 × 10<sup>−2</sup> mg dL<sup>−1</sup>. Additionally, CDs/GO NC exhibited outstanding consistency and specificity in recognizing CR within urine specimens from both healthy individuals and patients suffering from chronic kidney disease (CKD). The Bland–Altman assessment (utilizing 25 human urine specimens) displayed remarkable consensus (<i>R</i><sup>2</sup> = 0.995) among the FL approach and the benchmark Jaffe technique. This observation indicates the hands-on usefulness of the nanosensor for identifying CR in biological specimens. </p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Carbon dot-graphene oxide-based luminescent nanosensor for creatinine detection in human urine\",\"authors\":\"Poornima Bhatt, Deepak Kukkar, Ashok Kumar Yadav\",\"doi\":\"10.1007/s00604-024-06838-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A fluorescence (FL)-based nanosensor has been devised for creatinine (CR) detection in human urine specimens. The proposed nanosensor utilized a nanocomposite (NC) of carbon dots (CDs) and graphene oxide (GO). The formation of CDs/GO NC reduced the CD FL emission (<i>λ</i><sub>excitation</sub> = 390 nm, <i>λ</i><sub>emission</sub> = 461 nm) by ~ 75%. With the introduction of CR to the NC, the CD emission intensity was reinstated by approximately 70%. The linear detection range for CR was 10<sup>−5</sup> to 0.1 mg dL<sup>−1</sup> (<i>R</i><sup>2</sup> = 0.998), with a limit of detection of 4.3 × 10<sup>−2</sup> mg dL<sup>−1</sup>. Additionally, CDs/GO NC exhibited outstanding consistency and specificity in recognizing CR within urine specimens from both healthy individuals and patients suffering from chronic kidney disease (CKD). The Bland–Altman assessment (utilizing 25 human urine specimens) displayed remarkable consensus (<i>R</i><sup>2</sup> = 0.995) among the FL approach and the benchmark Jaffe technique. This observation indicates the hands-on usefulness of the nanosensor for identifying CR in biological specimens. </p><h3>Graphical abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"191 12\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-024-06838-8\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-024-06838-8","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Carbon dot-graphene oxide-based luminescent nanosensor for creatinine detection in human urine
A fluorescence (FL)-based nanosensor has been devised for creatinine (CR) detection in human urine specimens. The proposed nanosensor utilized a nanocomposite (NC) of carbon dots (CDs) and graphene oxide (GO). The formation of CDs/GO NC reduced the CD FL emission (λexcitation = 390 nm, λemission = 461 nm) by ~ 75%. With the introduction of CR to the NC, the CD emission intensity was reinstated by approximately 70%. The linear detection range for CR was 10−5 to 0.1 mg dL−1 (R2 = 0.998), with a limit of detection of 4.3 × 10−2 mg dL−1. Additionally, CDs/GO NC exhibited outstanding consistency and specificity in recognizing CR within urine specimens from both healthy individuals and patients suffering from chronic kidney disease (CKD). The Bland–Altman assessment (utilizing 25 human urine specimens) displayed remarkable consensus (R2 = 0.995) among the FL approach and the benchmark Jaffe technique. This observation indicates the hands-on usefulness of the nanosensor for identifying CR in biological specimens.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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