{"title":"从生乳和乳制品中分离出的耐甲氧西林金黄色葡萄球菌的流行率、抗菌药耐药性、毒力基因分布和 SCCmec 分型。","authors":"Najmeh Vahed Dehkordi, Ebrahim Rahimi, Noosha Zia Jahromi","doi":"10.18502/ijm.v16i5.16793","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>Researchers have focused on <i>Staphylococcus aureus</i> because it is transmitted through food, such as milk and dairy products, and causes human diseases. Prevalence, antimicrobial resistance, presence, and distribution of methicillin-resistant <i>S. aureus</i> (MRSA) virulence genes isolated from raw milk and dairy products were evaluated.</p><p><strong>Materials and methods: </strong>300 samples of dairy products were collected from Shahrekord, Iran. <i>S. aureus</i> was identified using biochemical tests and screened for sensitivity to 13 antibiotics to identify resistance genes. In addition, SCCmec typing was performed.</p><p><strong>Results: </strong>Out of 300, <i>S. aureus</i> was found in 82 samples. Raw milk had the highest contamination with <i>S. aureus</i> (60 of 82), followed by cheese (15 of 82), and butter (7 of 82). At least one resistance gene was present in every isolate of <i>S. aureus.</i> Virulence factors and enterotoxin-coding genes, such as <i>sea, seb, sec,</i> and <i>sed</i> were highly distributed.</p><p><strong>Conclusion: </strong>The results of this study revealed the presence of toxin-producing MRSA strains in raw milk and dairy products. MRSA in dairy farms is an important risk factor for the spread of staphylococcal infections; therefore, further studies are needed to find strategies for controlling the presence of <i>S. aureus</i>, especially MRSA, in dairy products.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"605-613"},"PeriodicalIF":1.3000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551661/pdf/","citationCount":"0","resultStr":"{\"title\":\"Prevalence, antimicrobial resistance, virulence gene distribution and SCCmec typing of methicillin-resistant <i>Staphylococcus aureus</i> isolated from raw milk and dairy products.\",\"authors\":\"Najmeh Vahed Dehkordi, Ebrahim Rahimi, Noosha Zia Jahromi\",\"doi\":\"10.18502/ijm.v16i5.16793\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>Researchers have focused on <i>Staphylococcus aureus</i> because it is transmitted through food, such as milk and dairy products, and causes human diseases. Prevalence, antimicrobial resistance, presence, and distribution of methicillin-resistant <i>S. aureus</i> (MRSA) virulence genes isolated from raw milk and dairy products were evaluated.</p><p><strong>Materials and methods: </strong>300 samples of dairy products were collected from Shahrekord, Iran. <i>S. aureus</i> was identified using biochemical tests and screened for sensitivity to 13 antibiotics to identify resistance genes. In addition, SCCmec typing was performed.</p><p><strong>Results: </strong>Out of 300, <i>S. aureus</i> was found in 82 samples. Raw milk had the highest contamination with <i>S. aureus</i> (60 of 82), followed by cheese (15 of 82), and butter (7 of 82). At least one resistance gene was present in every isolate of <i>S. aureus.</i> Virulence factors and enterotoxin-coding genes, such as <i>sea, seb, sec,</i> and <i>sed</i> were highly distributed.</p><p><strong>Conclusion: </strong>The results of this study revealed the presence of toxin-producing MRSA strains in raw milk and dairy products. MRSA in dairy farms is an important risk factor for the spread of staphylococcal infections; therefore, further studies are needed to find strategies for controlling the presence of <i>S. aureus</i>, especially MRSA, in dairy products.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"16 5\",\"pages\":\"605-613\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551661/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v16i5.16793\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v16i5.16793","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Prevalence, antimicrobial resistance, virulence gene distribution and SCCmec typing of methicillin-resistant Staphylococcus aureus isolated from raw milk and dairy products.
Background and objectives: Researchers have focused on Staphylococcus aureus because it is transmitted through food, such as milk and dairy products, and causes human diseases. Prevalence, antimicrobial resistance, presence, and distribution of methicillin-resistant S. aureus (MRSA) virulence genes isolated from raw milk and dairy products were evaluated.
Materials and methods: 300 samples of dairy products were collected from Shahrekord, Iran. S. aureus was identified using biochemical tests and screened for sensitivity to 13 antibiotics to identify resistance genes. In addition, SCCmec typing was performed.
Results: Out of 300, S. aureus was found in 82 samples. Raw milk had the highest contamination with S. aureus (60 of 82), followed by cheese (15 of 82), and butter (7 of 82). At least one resistance gene was present in every isolate of S. aureus. Virulence factors and enterotoxin-coding genes, such as sea, seb, sec, and sed were highly distributed.
Conclusion: The results of this study revealed the presence of toxin-producing MRSA strains in raw milk and dairy products. MRSA in dairy farms is an important risk factor for the spread of staphylococcal infections; therefore, further studies are needed to find strategies for controlling the presence of S. aureus, especially MRSA, in dairy products.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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